Cryptosporidium and Strongyloides stercoralis infections among people with and without HIV infection and efficiency of diagnostic methods for Strongyloides in Yirgalem Hospital, southern Ethiopia.
Cryptosporidium and Strongyloides stercoralis infections among people with and without HIV infection and efficiency of diagnostic methods for Strongyloides in Yirgalem Hospital, southern Ethiopia.
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DOI：
10.1186/1756-0500-3-90
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发表时间：
2010-04-01
影响因子：
1.8
通讯作者：
Shimelis T
中科院分区：
文献类型：
--
作者：
Getaneh A;Medhin G;Shimelis T
隐孢子虫病和圆线虫病已被报道与艾滋病毒/艾滋病有关。本研究旨在确定HIV感染者和非HIV感染者隐孢子虫和粪类圆线虫感染的流行情况，并对圆线虫的有效检测方法进行评估。2007年3月至2007年10月在埃塞俄比亚南部的Yirgalem医院进行了一项横断面研究。收集了384人（每个HIV血清组192人）的人口统计数据和粪便样本。采用改进的Ziehl-Neelsen技术对样品进行隐孢子虫种类的检测。粪便标本还采用直接盐垫法、甲醚法和水涌现法进行粪球菌诊断。HIV感染者中隐孢子虫和粪球菌的感染率分别为25%和12.0%。HIV阳性个体感染隐孢子虫（OR = 15.7; 95% CI 5.5 ~ 44.5）和粪球菌（OR = 6.4; 95% CI 2.2 ~ 18.9）的比例明显较高。三种诊断方法中，水出法检测粪虫幼虫的效率最高。在这项研究中，隐孢子虫和粪球菌感染的流行率在HIV/AIDS人群中明显较高。教育艾滋病毒感染者预防获得隐孢子虫感染和使用水涌现技术筛查粪球菌可能会有所帮助。
Cryptosporidiosis and strongyloidiasis have been reported to be associated with HIV/AIDS. The present study was designed to determine the prevalence of Cryptosporidium and Strongyloides stercoralis infections among people with and without HIV infection and also assess the efficient methods for detection of Strongyloides. A cross-sectional study was conducted in Yirgalem Hospital, southern Ethiopia from March, 2007 to October, 2007. Demographic data and stool samples were collected from 384 individuals (192 from each HIV serogroup). Samples were processed using the modified Ziehl-Neelsen technique for detection of Cryptosporidium species. Stool samples were also processed using the direct saline mount, the formol-ether and the water-emergence techniques for diagnosis of S. stercoralis. The prevalence of Cryptosporidium and S. stercoralis among HIV infected individuals was 25% and 12.0%, respectively. HIV positive individuals had significantly higher rate of infection with Cryptosporidium (OR = 15.7; 95% CI 5.5 to 44.5) and S. stercoralis (OR = 6.4; 95% CI 2.2 to 18.9). Among the three diagnostic methods, the larvae of S. stercoralis were more efficiently detected by the water-emergence technique. In this study, the prevalence of Cryptosporidium and S. stercoralis infections was significantly higher among people with HIV/AIDS. Educating HIV infected individuals to prevent acquisition of Cryptosporidium infection and screening for S. stercoralis using the water-emergence technique is likely to be helpful.