The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
复制标题
在杏鲍菇木质纤维素培养物中发现的新过氧化物酶的克隆以及与其他真菌过氧化物酶的序列比较。
DOI：
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发表时间：
2000
影响因子：
2.1
通讯作者：
Ángel T. Martínez
中科院分区：
文献类型：
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作者：
S. Camarero;F. J. Ruiz;Sovan Sarkar;María Jesús Martínez;Ángel T. Martínez
我们报告的基因ps1的克隆和测序编码一个多功能的过氧化物酶结合催化性能的木质素过氧化物酶（LiP）和锰过氧化物酶（MNP）分离的木质纤维素培养物的白腐真菌Pleumerynthalum。该基因含有15个内含子，推导的氨基酸序列由339个氨基酸残基的成熟蛋白和31个氨基酸残基的信号肽组成。在启动子区鉴定了几个推定的应答元件。参与Mn（2+）和芳香族底物氧化的氨基酸残基通过直接电子转移到血红素和从表面残基的远程电子转移，分别通过与黄孢原毛平革菌MnP和LiP的类比预测。一个树状图，说明29种真菌过氧化物酶之间的序列关系。
We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn(2+) and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.