Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.
Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.
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DOI：
10.1117/1.nph.10.3.035001
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发表时间：
2023-07
期刊：
影响因子：
5.3
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中科院分区：
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据推测，视网膜微循环异常可能预示大脑缺血性损伤的风险。使用类似的动物制剂和在类似的实验条件下对视网膜和大脑微循环进行直接比较将有助于验证这一假设。我们研究了控制条件下毛细血管红细胞（RBC）通量的变化和双侧颈动脉狭窄（BCAS）诱导的低灌注，然后将它们与我们之前在大脑中进行的测量结果进行了比较。我们使用荧光标记的红细胞传代方法，用双光子显微镜测量了小鼠视网膜的毛细血管红细胞通量。实验过程中监测关键生理参数，确保生理稳定。我们发现，在控制条件下，视网膜毛细血管红细胞通量远高于大脑（即大脑皮层灰质和皮层下白质），并且BCAS诱导视网膜毛细血管红细胞通量的下降幅度远大于大脑。我们展示了一种基于双光子显微镜的技术来有效地测量视网膜中的毛细血管红细胞通量。由于大脑皮层下白质经常由于整体灌注不足而表现出早期病理发展，我们的研究结果表明，视网膜微循环可能被用作涉及整体灌注不足的脑部疾病的早期标志。
It has been hypothesized that abnormal microcirculation in the retina might predict the risk of ischemic damages in the brain. Direct comparison between the retinal and the cerebral microcirculation using similar animal preparation and under similar experimental conditions would help test this hypothesis. We investigated capillary red-blood-cell (RBC) flux changes under controlled conditions and bilateral-carotid-artery-stenosis (BCAS)-induced hypoperfusion, and then compared them with our previous measurements performed in the brain. We measured capillary RBC flux in mouse retina with two-photon microscopy using a fluorescence-labeled RBC-passage approach. Key physiological parameters were monitored during experiments to ensure stable physiology. We found that under the controlled conditions, capillary RBC flux in the retina was much higher than in the brain (i.e., cerebral cortical gray matter and subcortical white matter), and that BCAS induced a much larger decrease in capillary RBC flux in the retina than in the brain. We demonstrated a two-photon microscopy-based technique to efficiently measure capillary RBC flux in the retina. Since cerebral subcortical white matter often exhibits early pathological developments due to global hypoperfusion, our results suggest that retinal microcirculation may be utilized as an early marker of brain diseases involving global hypoperfusion.