Soybean responds to phosphate starvation through reversible protein phosphorylation
Soybean responds to phosphate starvation through reversible protein phosphorylation
复制标题
大豆通过可逆蛋白质磷酸化应对磷酸盐饥饿
DOI：
10.1016/j.plaphy.2021.08.007
复制
发表时间：
2021-08-07
影响因子：
6.5
通讯作者：
Tian, Jiang
中科院分区：
文献类型：
--
作者：
Jiang, Weizhen;He, Panmin;Tian, Jiang
缺磷被认为是制约作物生产的主要因素。虽然大豆（甘氨酸max）对磷酸（Pi）缺乏的一系列适应性策略被广泛提出，但大豆对磷缺乏反应中可逆蛋白磷酸化的分子机制仍不清楚。本研究采用等压标签进行相对定量和绝对定量，结合液相色谱和串联质谱分析，鉴定了Pi充足和缺乏条件下大豆根系中的差异磷蛋白。共有427个磷酸化蛋白表现出不同的积累，其中213个蛋白表达上调，214个蛋白表达下调。其中，在低Pi条件下磷酸化水平升高的硝酸还原酶GmNR4被进一步选择，以评估磷酸化对其硝酸还原酶活性和亚细胞定位的影响。GmNR4磷酸化水平的突变显著影响其体外活性，但不影响其亚细胞定位。综上所述，鉴定的差异磷酸化蛋白揭示了大豆通过可逆蛋白磷酸化适应Pi饥饿的复杂调控途径。
Phosphorus (P) deficiency is considered as a major constraint on crop production. Although a set of adaptative strategies are extensively suggested in soybean (Glycine max) to phosphate (Pi) deprivation, molecular mechanisms underlying reversible protein phosphorylation in soybean responses to P deficiency remains largely unclear. In this study, isobaric tags for relative and absolute quantitation, combined with liquid chromatography and tandem mass spectrometry analysis was performed to identify differential phosphoproteins in soybean roots under Pi sufficient and deficient conditions. A total of 427 phosphoproteins were found to exhibit differential accumulations, with 213 up-regulated and 214 down-regulated. Among them, a nitrate reductase, GmNR4 exhibiting increased phosphorylation levels under low Pi conditions, was further selected to evaluate the effects of phosphorylation on its nitrate reductase activity and subcellular localization. Mutations of GmNR4 phosphorylation levels significantly influenced its activity in vitro, but not for its subcellular localization. Taken together, identification of differential phosphoproteins reveled the complex regulatory pathways for soybean adaptation to Pi starvation through reversible protein phosphorylation.