Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone
Raman spectroscopic imaging markers for fatigue-related microdamage in bovine bone
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DOI：
10.1021/ac9913560
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发表时间：
2000-05-15
影响因子：
7.4
通讯作者：
Kohn, DH
中科院分区：
文献类型：
--
作者：
Timlin, JA;Garden, A;Kohn, DH
拉曼光谱标记物已被确定为牛骨疲劳相关的微损伤。微损伤是在循环疲劳加载条件下产生的。加载后，用碱性品红对标本进行整体染色，以便于显示损伤并区分疲劳损伤和随后的组织切片中产生的裂纹。对骨组织标本进行光学显微镜和高光谱近红外拉曼成像显微镜检查，确定三个区域：无可见损伤的组织、有微裂纹的组织和弥漫性损伤的组织。拉曼横断面是150-200条拉曼光谱的线条，用于最初的组织调查。横断面拉曼光谱的探索性因子分析已经确定了与损伤相关的骨标本的光谱明显的化学微结构。在选定的损伤区域，获得了1.4微米空间分辨率的全高光谱拉曼图像。在未受损的组织区域，磷酸VL带在957厘米(-1)处被发现，正如碳化的羟基磷灰石骨矿所预期的那样。然而，在可见的微损伤区域，在963厘米(-1)处观察到一个额外的磷酸盐Nu(1)带，并被解释为一种更化学计量比的、较少碳酸盐的矿物物种。拉曼成像证实了骨矿物质的拉曼光谱特征与牛骨微损伤类型之间的定性关系。对受损区域碳酸盐含量较低的原因提出了两种尝试性的解释。
Raman spectroscopic markers have been determined for fatigue-related microdamage in bovine bone. Microdamage was induced using a cyclic fatigue loading regime. After loading, the specimens were stained en-bloc with basic fuchsin to facilitate damage visualization and differentiate fatigue-induced damage from cracks generated during subsequent histological sectioning, Bone tissue specimens were examined by light microscopy and hyperspectral near-infrared Raman imaging microscopy, Three regions were defined-tissue with no visible damage, tissue with microcracks, and tissue with diffuse damage. Raman transects, lines of 150-200 Raman spectra, were used for initial tissue surveys. Exploratory factor analysis of the transect Raman spectra has identified spectroscopically distinct chemical microstructures of the bone specimens that correlate with damage. In selected regions of damage, full hyperspectral Raman images were obtained with 1.4-mu m spatial resolution. In regions of undamaged tissue, the phosphate vl band is found at 957 cm(-1), as expected for the carbonated hydroxyapatic bone mineral. However, in regions of visible microdamage, an additional phosphate nu(1) band is observed at 963 cm(-1) and interpreted as a more stoichiometric, less carbonated mineral species. Raman imaging confirms the qualitative relationship between the Raman spectral signature of bone mineral and the type of microdamage in bovine bone. Two tentative explanations for the presence of less carbonated phosphate in damaged regions are proposed.