Expression and Purification of Cyanobacterial Circadian Clock Protein KaiC and Determination of Its Auto-phosphatase Activity.
Expression and Purification of Cyanobacterial Circadian Clock Protein KaiC and Determination of Its Auto-phosphatase Activity.
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DOI：
10.21769/bioprotoc.2140
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发表时间：
2017-02
期刊：
影响因子：
0.8
通讯作者：
Qiang Chen;Ling-ling Yu;X. Tan;Sen Liu
中科院分区：
文献类型：
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作者：
Qiang Chen;Ling-ling Yu;X. Tan;Sen Liu
昼夜节律是一种周期约为24 h的内源性振荡的生物学过程。它们使生物体能够预测并为主要由地球自转引起的环境变化做好准备。昼夜节律是由蛋白质、DNA和/或RNA组成的生物钟驱动的。蓝藻的生物钟是最简单的，也是研究得最好的模型之一。它们含有三种时钟蛋白KaiA、KaiB和KaiC，可用于体外重建实验和测定KaiC的自身磷酸酶活性，如本方案所述。
Circadian rhythms are biological processes displaying an endogenous oscillation with a period of ~24 h. They allow organisms to anticipate and get prepared for the environmental changes caused mainly by the rotation of Earth. Circadian rhythms are driven by circadian clocks that consist of proteins, DNA, and/or RNA. Circadian clocks of cyanobacteria are the simplest and one of the best studied models. They contain the three clock proteins KaiA, KaiB, and KaiC which can be used for in vitro reconstitution experiments and determination of the auto-phosphatase activity of KaiC as described in this protocol.