Investigations using immunization to attenuate the psychoactive effects of nicotine
Investigations using immunization to attenuate the psychoactive effects of nicotine
复制标题
DOI：
10.1016/j.bmc.2003.11.029
复制
发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Janda, KD
中科院分区：
文献类型：
--
作者：
Carrera, MRA;Ashley, JA;Janda, KD
尽管存在巨大的健康风险，但人们继续吸烟和使用烟草主要是由于尼古丁成瘾。作为免疫药物治疗研究的一部分，我们研究了主动免疫和被动免疫对大鼠急性尼古丁诱导的运动活性的影响。为此，在一系列尼古丁挑战和测试之前，给大鼠接种旨在引发抗尼古丁免疫反应的NIC-KLH免疫偶联疫苗，或注射抗尼古丁单克隆抗体NIC9D9。接种疫苗的大鼠的运动活动减少了45%，而对照组减少了16%。NIC9D9被动免疫导致运动活动减少66.9%，而对照组减少3.4%。与行为数据一致，免疫大鼠大脑中发现的尼古丁要少得多。该结果支持在戒烟计划背景下免疫药物治疗尼古丁成瘾的潜在临床价值。(C) 2003 Elsevier Ltd.版权所有。
Despite the enormous health risks, people continue to smoke and use tobacco primarily as a result of nicotine addiction. As part of our immunopharmacotherapy research, the effects of active and passive immunizations on acute nicotine-induced locomotor activity in rats were investigated. To this end, rats were immunized with either a NIC-KLH immunoconjugate vaccine designed to elicit an antinicotine immune response, or were administered an antinicotine monoclonal antibody, NIC9D9, prior to a series of nicotine challenges and testing sessions. Vaccinated rats showed a 45% decrease in locomotor activity compared to a 16% decrease in controls. Passive immunization with NIC9D9 resulted in a 66.9% decrease in locomotor activity versus a 3.4% decrease in controls. Consistent with the behavioral data, much less nicotine was found in the brains of immunized rats. The results support the potential clinical value of immunopharmacotherapy for nicotine addiction in the context of tobacco cessation programs. (C) 2003 Elsevier Ltd. All rights reserved.