The photo-excited triplet state of chlorophyll d in methyl-tetrahydrofuran studied by optically detected magnetic resonance and time-resolved EPR
The photo-excited triplet state of chlorophyll d in methyl-tetrahydrofuran studied by optically detected magnetic resonance and time-resolved EPR
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DOI：
10.1080/00268970701627797
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Carbonera, Donatella
中科院分区：
文献类型：
--
作者：
Di Valentin, Marilena;Ceola, Stefano;Carbonera, Donatella
叶绿素d(Chl D)是海洋绿光细菌(Acaryo氯is marina)光系统(PSI和II)中的主要色素。这一事实表明，基于Chl d而不是Chl a的光合作用可能是氧合光合作用中一种有趣的选择。虽然在体内和体外都有大量与Chla相关的光谱信息，但关于Chl d的文献很少。特别是，Chl d的三联体状态到目前为止还没有在体外进行研究。虽然三重态并不代表光合作用过程中的主要激发途径，但它们参与了天线复合体和反应中心的光胁迫事件，也可以用作分子环境的内源顺磁探针。本文首次利用时间分辨EPR和ODMR表征了极性溶剂甲基四氢呋喃中的Chl-d三重态。并与在相同实验条件下测得的Chla光谱进行了比较。
Chlorophyll d (Chl d) is the major pigment in the antenna proteins of both photosystems (PSI and II) of the oxyphotobacterium Acaryochloris marina. This fact suggests that photosynthesis based upon Chl d rather than Chl a may be an interesting alternative in oxygenic photosynthesis. While a great deal of spectroscopic information relative to Chl a is available, both in vivo and in vitro, the literature on Chl d is scarce. In particular, the triplet state of Chl d has not been studied in vitro to date. Although triplet states do not represent the main excitation path in the photosynthetic process, they are involved in light stress events both in the antenna complexes and in the reaction centers and may also be used as endogenous paramagnetic probes of the molecular environment. In this paper we make use of both time-resolved EPR and ODMR to characterize, for the first time, the Chl d triplet state in the polar solvent methyl-tetrahydrofuran. Comparison with the spectra of Chl a obtained under the same experimental conditions is also discussed.