CYTOPLASMIC STRUCTURES ASSOCIATED WITH AN ARBOVIRUS INFECTION - LOCI OF VIRAL RIBONUCLEIC ACID SYNTHESIS
CYTOPLASMIC STRUCTURES ASSOCIATED WITH AN ARBOVIRUS INFECTION - LOCI OF VIRAL RIBONUCLEIC ACID SYNTHESIS
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DOI：
10.1128/jvi.2.11.1326-1338.1968
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发表时间：
1968-01-01
影响因子：
5.4
通讯作者：
FRIEDMAN, RM
中科院分区：
文献类型：
--
作者：
GRIMLEY, PM;BEREZESKY, IK;FRIEDMAN, RM
在塞姆利基森林病毒复制的对数期早期的鸡胚细胞中发现了独特的细胞质结构，本文称为 I 型细胞病变空泡 (CPV-I)。高分辨率放射自显影证明CPV-I是3 H-尿苷掺入的位点。这一证据与先前的生化数据和塞姆利基森林病毒核糖核酸合成中活跃的亚细胞部分的电子显微镜观察结果很好地相关。通过电子致密示踪粒子的分布和连续的超微结构观察证实了宿主细胞细胞质内 CPV-I 的起源。
Unique cytoplasmic structures, herein designated as type I cytopathic vacuoles (CPV-I), are found in chick embryo cells early in the logarithmic phase of Semliki Forest virus replication. High resolution autoradiography demonstrated that the CPV-I are loci of3H-uridine incorporation. This evidence correlates well with previous biochemical data and electron microscopy of the subcellular fractions active in Semliki Forest virus ribonucleic acid synthesis. Origin of the CPV-I within host cell cytoplasm is confirmed by the distribution of electron-dense tracer particles and sequential ultrastructural observations.