A broad exploration of a transgenic population of citrus:: stability of gene expression and phenotype
A broad exploration of a transgenic population of citrus:: stability of gene expression and phenotype
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DOI：
10.1007/s001220051338
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发表时间：
2000-03-01
影响因子：
5.4
通讯作者：
Peña, L
中科院分区：
文献类型：
--
作者：
Cervera, M;Pina, JA;Peña, L
收集了70株uidA和npt II基因转基因柑橘植物，在温室条件下保存了4-5年。详细扫描的植物，使我们能够检测到四个表型的异型植物和一个大的变化的转基因整合和表达模式之间的人口。对异型植株进行了分析，其特征在于珠心四倍体，可能源自四倍体起始组织，而不是体细胞克隆变异事件。转基因整合和表达分析表明：（1）拷贝数与GUS活性之间存在显著的负相关;（2）T-DNA插入片段的重排并不意味着低表达水平;（3）在自然环境条件下生长的所有转化体都证实了转基因整合和表达的稳定性。这些组合特征验证了转化作为柑橘遗传改良的工具。
A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus.