Subcellular dynamics of red clover necrotic mosaic virus double-stranded RNAs in infected plant cells
Subcellular dynamics of red clover necrotic mosaic virus double-stranded RNAs in infected plant cells
复制标题
受感染植物细胞中红三叶草坏死花叶病毒双链RNA的亚细胞动力学
DOI：
10.1016/j.virol.2022.01.015
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Masanori Kaido
中科院分区：
文献类型：
--
作者：
Shota Takata;Kazuyuki Mise;Yoshitaka Takano;Masanori Kaido
新的证据表明，病毒复制复合体（VRCs）不仅在病毒复制中发挥重要作用，而且在病毒细胞间运动中也发挥重要作用。目前，VRCs如何在大小上生长并与病毒运动蛋白（MPs）共定位仍不清楚。本研究利用报告基因B2-GFP植物对红三叶草坏死花叶病毒（RCNMV）的dsRNA进行了活细胞成像。在感染早期沿内质网（ER）形成微小的dsRNA颗粒。重要的是，dsRNA颗粒与病毒编码的p27复制蛋白的共定位表明，这些结构是VRCs的组成部分。这些颗粒在肌动蛋白系统的驱动下在细胞质中移动，并相互结合形成更大的聚集体；MPs与这些过程无关。值得注意的是，MPs优先与大的dsRNA聚集体而不是小的dsRNA颗粒共定位，这表明VRCs大小的增加促进了它们与MPs的共定位。
New evidences are emerging to support the importance of viral replication complexes (VRCs) in not only viral replication, but also viral cell-to-cell movement. Currently, how VRCs grow in size and colocalize with viral movement proteins (MPs) remains unclear. Herein, we performed live-cell imaging of red clover necrotic mosaic virus (RCNMV) dsRNA by using reporter B2-GFP plants. Tiny granules of dsRNA were formed along the endoplasmic reticulum (ER) at an early stage of infection. Importantly, the colocalization of the dsRNA granules with the virus-encoded p27 replication protein showed that these structures are components of VRCs. These granules moved throughout the cytoplasm, driven by the acto–myosin system, and coalesced with each other to form larger aggregates; the MPs were not associated with these processes. Notably, the MPs colocalized preferentially with large dsRNA aggregates, rather than with tiny dsRNA granules, suggesting that the increase in the size of VRCs promotes their colocalization with MPs.