DIFFERENT EXPRESSION TIME OF THE 105-KDA PROTEIN AND 90-KDA HEAT-SHOCK PROTEIN IN RAT TESTIS
DIFFERENT EXPRESSION TIME OF THE 105-KDA PROTEIN AND 90-KDA HEAT-SHOCK PROTEIN IN RAT TESTIS
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DOI：
10.1016/0014-5793(91)80920-x
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发表时间：
1991-09-02
期刊：
影响因子：
3.5
通讯作者：
TASHIMA, Y
中科院分区：
文献类型：
--
作者：
ITOH, H;TASHIMA, Y
为了了解睾丸特异性的105-kDa蛋白和HSP 90（90-kDa热休克蛋白）相关蛋白的生理功能，在大鼠发育过程中跟踪了其在睾丸中的出现。 在免疫印迹分析中，105 kDa蛋白直到5周龄后才出现，而HSP 90在3周龄时就可以检测到。 在精子中，105-kDa的蛋白质是非常丰富的，但不是在LC-540细胞（大鼠睾丸间质细胞瘤的细胞系）胞质溶胶。 这一发现引起了人们对这种蛋白质与精子功能之间关系的广泛关注。
To understand the physiological functions of the 105-kDa protein which is testis-specific and HSP90 (90-kDa heat-shock protein) related protein, the appearance of it in the testis has been followed during the development of rat. On immunoblotting analysis, the 105-kDa protein did not appear until after the age of five weeks, while HSP90 could be detected at three weeks. In the spermatozoa, the 105-kDa protein was much abundant but not in the LC-540 cells (a cell line from Leydig cell tumor in rat testis) cytosol. This finding has attracted much attention to the relationship between this protein and sperm functions.