Hepatocyte-derived microRNAs as serum biomarkers of hepatic injury and rejection after liver transplantation
Hepatocyte-derived microRNAs as serum biomarkers of hepatic injury and rejection after liver transplantation
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DOI：
10.1002/lt.22438
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发表时间：
2012-03-01
影响因子：
4.6
通讯作者：
van der Laan, Luc J. W.
中科院分区：
文献类型：
--
作者：
Farid, Waqar R. R.;Pan, Qiuwei;van der Laan, Luc J. W.
最近的动物和人类研究强调了血清中肝细胞衍生的microRNA（HDmiR）作为肝损伤的早期、稳定、敏感和特异性生物标志物的潜力。然而，它们在人类肝移植中的有用性尚未得到解决。本研究旨在探讨血清HDmiR作为肝移植中肝损伤和排斥反应的标志物。通过HDmiR（miR-122、miR-148 a和miR-194）的实时聚合酶链反应定量分析来自健康对照和肝移植受者（n = 107）的血清样品和移植物周围肝移植物活检样品（n = 45）。随着移植物热缺血时间的延长，肝组织中miR-122和miR-148 a的表达显著降低。相反，这些HDmiR的血清水平在肝损伤患者中升高，并与转氨酶水平呈正相关。HDmiR似乎非常敏感，因为转氨酶值正常的患者（
Recent animal and human studies have highlighted the potential of hepatocyte-derived microRNAs (HDmiRs) in serum as early, stable, sensitive, and specific biomarkers of liver injury. Their usefulness in human liver transplantation, however, has not been addressed. The aim of this study was to investigate serum HDmiRs as markers of hepatic injury and rejection in liver transplantation. Serum samples from healthy controls and liver transplant recipients (n = 107) and peritransplant liver allograft biopsy samples (n = 45) were analyzed via the real-time polymerase chain reaction quantification of HDmiRs (miR-122, miR-148a, and miR-194). The expression of miR-122 and miR-148a in liver tissue was significantly reduced with prolonged graft warm ischemia times. Conversely, the serum levels of these HDmiRs were elevated in patients with liver injury and positively correlated with aminotransferase levels. HDmiRs appear to be very sensitive because patients with normal aminotransferase values (