Mitochondrial DNA sequence data indicate evidence for multiple species within Peromyscus maniculatus
Mitochondrial DNA sequence data indicate evidence for multiple species within Peromyscus maniculatus
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线粒体 DNA 序列数据表明了 Peromyscus maniculatus 中存在多个物种的证据
DOI：
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
L. L. Lindsey
中科院分区：
文献类型：
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作者：
R. Bradley;L. L. Lindsey
Peromyscus maniculatus 是北美最常见和最深入研究的小型哺乳动物物种之一。由于广泛的地理分布（加拿大到墨西哥中部）、相对大量的形态和遗传变异以及大量的分类学历史，该分类单元的分类界限很难辨别。在此，进行了一项全面的系统发育研究，以评估整个 P. maniculatus 范围内的遗传变异模式，以解决分类问题并确定系统群的分布。线粒体细胞色素-b 基因的 DNA 序列来自 681 个个体，代表正式分配给 P. maniculatus 的 68 个公认亚种中的 28 个，以及分配给 P. maniculatus 物种组的所有成员，以评估广泛地理范围内的遗传变异。进行简约、贝叶斯和最大似然分析以确定系统发育关系；此外，对遗传距离和分化日期进行了近似，以帮助确定系统群的起源时间。这些分析的结果揭示了八个主要系统发育群体的存在。除了一个例外，每个谱系似乎都代表一个独特的系统发育群体，对应于一个不同的地理区域。结果与其他研究一致，表明 P. maniculatus (sensu lato) 包含多个系统群。系统群的识别以及分类学优先原则的应用表明：1）来自内华达州西部、加利福尼亚州南部和下加利福尼亚州的样本应称为Peromyscus gambelii； 2) 来自育空地区西南部的样本应称为 P. sp.； 3) 来自阿拉斯加东南部和育空地区中南部，沿不列颠哥伦比亚省沿海向南至华盛顿州西南部的样本应称为 P.ukeini； 4）来自北美中西部的样本应称为Peromyscus sonoriensis； 5) 来自加拿大东部和美国东部的样品应称为P. maniculatus； 6) 来自新墨西哥州西南部和墨西哥中部的样本应称为 Peromyscus labecula； 7) 来自墨西哥南巴哈圣克鲁斯岛的 Peromyscus sejugis 应暂时保留为有效物种名称。这七个物种与 Peromyscus melanotis 和 Peromyscus poliionotus 一起组成了 P. maniculatus 物种组。
Peromyscus maniculatus is one of the most commonly encountered and intensively studied small mammal species in North America. Because of a broad geographic distribution (Canada to central Mexico), relatively large amounts of morphometric and genetic variation, and a voluminous taxonomic history, the taxonomic delimitations of this taxon have been difficult to discern. Herein, a comprehensive phylogenetic study was conducted to assess patterns of genetic variation throughout the range of P. maniculatus to resolve the taxonomy and ascertain the distribution of phylogroups. DNA sequences from the mitochondrial cytochrome-b gene were included from 681 individuals, representing 28 of the 68 recognized subspecies formally assigned to P. maniculatus, as well as all members assigned to the P. maniculatus species group, to assess genetic variation across a broad geographic context. Parsimony, Bayesian, and maximum likelihood analyses were conducted to determine phylogenetic relationships; further, genetic distances and divergence dates were approximated to assist in determining the time of origin for phylogroups. Results of these analyses reveal the presence of eight major phylogenetic groups. With one exception, each lineage appears to represent a unique phylogenetic group that corresponds to a distinct geographic region. Results are in agreement with other studies suggesting that P. maniculatus (sensu lato) contained multiple phylogroups. Recognition of phylogroups followed by the application of the principals of taxonomic priority indicate that: 1) samples from western Nevada, southern California, and Baja California should be referred to as Peromyscus gambelii; 2) samples from the southwestern Yukon should be referred to as P. sp.; 3) samples from southeastern Alaska and southcentral Yukon southward along coastal British Columbia to southwestern Washington should be referred to as P. keeni; 4) samples from central and western North America should be referred to as Peromyscus sonoriensis; 5) samples from eastern Canada and the eastern United States should be referred to as P. maniculatus; 6) samples from extreme southwestern New Mexico and central Mexico should be referred to as Peromyscus labecula; and 7) Peromyscus sejugis from Isla Santa Cruz, Baja del Sur, Mexico, should be tentatively retained as a valid species name. These seven species along with Peromyscus melanotis and Peromyscus polionotus comprise the P. maniculatus species group.