Anti-inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract
Anti-inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract
复制标题
淫羊藿乙醇提取物的抗炎活性
DOI：
10.1089/jmf.2017.4088
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发表时间：
2018-04-05
影响因子：
2.4
通讯作者：
Li, Bin
中科院分区：
文献类型：
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作者：
Huang, Shan;Meng, Ning;Li, Bin
淫羊藿是我国传统的中草药。本研究观察了E.在用脂多糖（LPS）攻击的RAW 264.7巨噬细胞和小鼠中研究了短角芹乙醇提取物（EBME）。结果显示，EBME通过减少LPS刺激的RAW 264.7巨噬细胞中几种促炎介质如一氧化氮（NO）、前列腺素（PG）E-2、诱导型一氧化氮合酶和环氧合酶-2的产生来减轻炎症。EBME增加血红素氧合酶-1（HO-1）的表达，促进核因子红细胞2相关因子2的核转位。EBME对LPS刺激的NO和PGE（2）表达的抑制作用可被HO-1抑制剂部分逆转。EBME还通过抑制LPS诱导的腹膜炎中肿瘤坏死因子-、白细胞介素（IL）-1和IL-6的产生而引起抗炎作用。因此，EBME在体外和体内均表现出抗炎作用。
Epimedium brevicornu Maxim has been used as a traditional herbal drug in China. In this study, the anti-inflammatory effects of E. brevicornu Maxim ethanol extract (EBME) were investigated in RAW264.7 macrophages and mice challenged with lipopolysaccharide (LPS). Results showed that EBME attenuated inflammation by decreasing the production of several proinflammatory mediators, such as nitric oxide (NO), prostaglandin (PG) E-2, inducible nitric oxide synthase, and cyclooxygenase-2, in LPS-stimulated RAW264.7 macrophages. EBME increased the expression of heme oxygenase-1 (HO-1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2. The inhibitory effects of EBME on LPS-stimulated NO and PGE(2) expression were partially reversed by HO-1 inhibitor. EBME also elicited an anti-inflammatory effect by inhibiting the production of tumor necrosis factor-, interleukin (IL)-1, and IL-6 in LPS-induced peritonitis. Therefore, EBME exhibited anti-inflammatory effects in vitro and in vivo.