Combining growth factor releasing microspheres within aligned nanofibers enhances neurite outgrowth.
Combining growth factor releasing microspheres within aligned nanofibers enhances neurite outgrowth.
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DOI：
10.1002/jbm.a.36204
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Tonya J Whitehead;Christian Oliver C. Avila;H. Sundararaghavan
中科院分区：
文献类型：
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作者：
Tonya J Whitehead;Christian Oliver C. Avila;H. Sundararaghavan
目前周围神经损伤的治疗包括自体移植物、金标准和市售的神经生长导管（NGCs）。自体移植物有几个缺点，包括供体部位发病率和神经大小不匹配，导致不完全恢复。然而，即使存在这些缺陷，自体移植物也比缺乏足够线索促进完全再生的市售NGCs工作得更好。本研究评估了可添加到NGC中空内部空间的生物材料成分组合，以促进和指导神经再生；具体来说，是机械的、化学的和地形的线索。甲基丙烯酸透明质酸（MeHA，机械线索）被静电纺成排列的纤维（地形线索），与聚乳酸-羟基乙酸微球一起输送神经生长因子（NGF，化学线索）。采用环境扫描电镜和力学性能测试对支架在生理条件下的性能进行了评价。所得支架的水合孔隙率为35-55%，杨氏模量为0.43-2.86 MPa。酶联免疫吸附试验表明，NGF从微球中释放长达4周。背根神经节（DRG）神经元显示释放的NGF具有生物活性。支架上的DRG测试也表明，微球释放的NGF与排列的纳米纤维结合可以显著促进神经突的生长。©2017 Wiley期刊公司[J] .生物医学工程学报，2016,31(4):557 - 557。
Current treatments for peripheral nerve injuries include autografts, the gold standard, and commercially available nerve growth conduits (NGCs). Autografts have several drawbacks including donor site morbidity and nerve size mismatch, which lead to incomplete recovery. However, even with these drawbacks, autografts work better then commercially available NGCs that lack sufficient cues to promote complete regeneration. This study evaluated a combination of biomaterial components that can be added to the hollow internal space of a NGC to promote and direct nerve regeneration; specifically, mechanical, chemical, and topographical cues. Methacrylated hyaluronic acid (MeHA, mechanical cue) is electrospun into aligned fibers (topographical cue), with poly-lactic-co-glycolic acid microspheres to deliver nerve growth factor (NGF, chemical cue). The properties of the scaffold were evaluated under physiological conditions using environmental scanning electron microscopy and mechanical testing. The resulting scaffolds have hydrated porosities of 35-55% and Young's modulus in the range of 0.43-2.86 MPa. Enzyme-linked immunosorbent assay showed that NGF is released from the microspheres for up to 4 weeks. Dorsal root ganglia (DRG) neurons showed that the released NGF is bioactive. DRG testing on the scaffolds also showed that the combination of NGF released from the microspheres and the aligned nanofibers significantly enhanced neurite outgrowth. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 17-25, 2018.