Identifying four Trypanosoma cruzi I isolate haplotypes from different geographic regions in Colombia
Identifying four Trypanosoma cruzi I isolate haplotypes from different geographic regions in Colombia
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DOI：
10.1016/j.meegid.2006.12.003
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Guhl, Felipe
中科院分区：
文献类型：
--
作者：
Herrera, Claudia;Bargues, M. Dolores;Guhl, Felipe
克氏锥虫被分为克氏锥虫I和克氏锥虫II两组。根据多位点酶电泳和随机扩增多态性DNA，后者被细分为5个较小的谱系，命名为IIa-IIe，这表明与rRNA/miniexon谱系相对应。12个以前的特点克氏锥虫分离株从不同的主机，包括人类，Didelphis marsupialis，锥虫进行了分析，以建立GE变异克氏锥虫组克氏锥虫我分离株从不同的地理区域的哥伦比亚。DNA样本基于mini-exon基因间隔区进行测序。使用Clustal W、Staden 1.5和MEGA 3软件分析序列，并使用来自GenBank的报道序列作为参考。采用Kimura的双参数模型对遗传距离进行了分析。核苷酸序列的联合比对结果为350 bp，计算的核苷酸差异为17.5%。差异包括23个转换（7.2%）、14个颠换（4.4%）和19个插入-缺失（5.9%）。哥伦比亚T critzi I分离株揭示了足够的遗传变异性，我们提出存在四个单倍型，通过单核苷酸多态性（SNP）和插入/缺失发现的小外显子基因的非转录间隔基因间区域。(c)2007年由Elsevier B. V.出版。
Trypanosoma cruzi has been classified into the groups T cruzi I and T cruzi II. The latter is subdivided into five smaller lineages based on multilocus enzyme electrophoresis and random amplified polymorphic DNA, designated as IIa-IIe, which shows correspondence with rRNA/miniexon lineages. Twelve previously characterised T cruzi isolates from different hosts, including humans, Didelphis marsupialis, and triatomines were analysed to establish ge variability in T cruzi group T cruzi I isolates from different geographical regions of Colombia. DNA samples were sequenced based oil the mini-exon gene intergenic region. Sequences were analysed using Clustal W, Staden 1.5 and MEGA3 software, and using reported sequences from the GenBank as reference. The genetic distances were analysed using Kimura's two-parameter model. The isolates ' joint alignment was of 350 bp, and the calculated nucleotide divergence was of 17.5%. The differences consisted of 23 transitions (7.2%) 14 transversions (4.4%) and 19 insertion-deletions (5.9%). The Colombian T critzi I isolates revealed sufficient genetic variability for us to propose the existence of four haplotypes identified through single nucleotide polymorphism (SNP) and insertion/deletion found in the mini-exon Gene's non-transcribed spacer intergenic region. (c) 2007 Published by Elsevier B.V.