Regional differences in adipose tissue metabolism in obese men
Regional differences in adipose tissue metabolism in obese men
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DOI：
10.1016/j.metabol.2006.11.015
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发表时间：
2007-04-01
影响因子：
9.8
通讯作者：
Tchernof, Andre
中科院分区：
文献类型：
--
作者：
Boivin, Ariane;Brochu, Gaetan;Tchernof, Andre
我们检测了33名男性的网膜和皮下脂肪组织脂肪细胞大小、脂解和脂蛋白脂酶（LPL）活性，这些男性年龄在22.6至61.2岁之间，体重指数范围为24.6至79.1 kg/m2。我们检验了网膜脂肪库的脂解率高于皮下脂肪组织的假设，并且这种差异在腹部肥胖值的范围内持续存在。在手术过程中获得网膜和皮下脂肪组织样本。胶原酶消化法分离脂肪细胞。脂肪细胞的大小和LPL活性以及基础，异丙肾上腺素，毛喉素，和二丁酰环磷酸腺苷刺激的脂解进行了测定。虽然肥胖者的两个脂肪室的脂肪细胞都较大，但网膜脂肪细胞与皮下脂肪细胞的大小没有差异。脂蛋白脂酶活性，作为细胞数量的函数，网膜脂肪组织显著高于皮下脂肪组织（P <0.005）。基础脂解和脂解反应异丙肾上腺素，毛喉素，或二丁酰环磷酸腺苷，无论是作为细胞数量的函数或作为一个倍数的基础水平的反应，网膜与皮下脂肪细胞没有显着差异。当以腰围的三分位数对样本进行分层时，对于所有肥胖值，网膜和皮下贮库中的脂肪细胞直径相似。网膜脂肪细胞大小在腰围的2个上三分位数中达到平台，即从腰围125 cm及以上开始。脂蛋白脂肪酶活性在腰围中三分位数的网膜细胞中显著较高（P = 0.05），并且在腰围三分位数的脂肪分解值中没有发现区域差异。总之，在正常体重至病态肥胖的男性中，虽然脂肪细胞大小和脂解倾向于随着腰围的增加而增加，但在网膜和皮下脂肪库之间没有观察到差异。(c)2007年爱思唯尔公司All rights reserved.
We examined omental and subcutaneous adipose tissue adipocyte size, and lipolysis and lipoprotein lipase (LPL) activity in a sample of 33 men aged 22.6 to 61.2 years and with a body mass index ranging from 24.6 to 79.1 kg/m(2). We tested the hypothesis that lipolysis rates would be higher in the omental fat depot than in subcutaneous adipose tissue and that this difference would persist across the spectrum of abdominal adiposity values. Omental and subcutaneous adipose tissue samples were obtained during surgery. Adipocytes were isolated by collagenase digestion. Adipocyte size and LPL activity as well as basal, isoproterenol-, forskolin-, and dibutyryl cyclic adenosine monophosphate-stimulated lipolysis were measured. Although adipocytes from both fat compartments were larger in obese subjects, no difference was observed in the size of omental vs subcutaneous fat cells. Lipoprotein lipase activity, expressed as a function of cell number, was significantly higher in omental than in subcutaneous fat tissue (P < .005). Basal lipolysis and lipolytic responses to isoproterenol, forskolin, or dibutyryl cyclic adenosine monophosphate, expressed either as a function of cell number or as a fold response over basal levels, were not significantly different in omental vs subcutaneous fat cells. When stratifying the sample in tertiles of waist circumference, adipocyte diameter was similar in the omental and subcutaneous depots for all adiposity values. Omental adipocyte size reached a plateau in the 2 upper tertiles of waist circumference, that is, from a waist circumference of 125 cm and above. Lipoprotein lipase activity was significantly higher in omental cells in the middle tertile of waist circumference (P = .05), and no regional difference was noted in lipolysis values across waist circumference tertiles. In conclusion, in normal-weight to morbidly obese men, although adipocyte size and lipolysis tended to increase with higher waist circumference, no difference was observed between the omental and subcutaneous fat depot. (c) 2007 Elsevier Inc. All rights reserved.