Molecularly imprinted polyurethane grafted calcium alginate hydrogel with specific recognition for proteins
Molecularly imprinted polyurethane grafted calcium alginate hydrogel with specific recognition for proteins
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DOI：
10.1016/j.matlet.2014.12.108
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发表时间：
2015-03-15
影响因子：
3
通讯作者：
Zhang, Weiying
中科院分区：
文献类型：
--
作者：
Li, Lingxie;Ying, Xiaoguang;Zhang, Weiying
制备了用于牛血清白蛋白印迹的聚氨脂接枝海藻酸钙水凝胶微球，并对其进行了表征。接枝修饰是在蛋白质模板与水凝胶组装之前进行的，这有利于维持蛋白质的构象和构建更容易接近的印迹位点。印迹水凝胶经修饰后对模板分子具有较高的亲和力。接枝率对特异性重结合和竞争性质影响的研究表明，接枝侧链有利于蛋白质的选择性识别。(C) 2014 Elsevier B.V.版权所有
Polyurethane grafted calcium alginate hydrogel microspheres intended for bovine serum albumin imprinting were prepared and characterized. The grafting modification was performed prior to the assembling of protein template with hydrogel, which was advantageous to maintain protein conformation and construct more accessible imprinted sites. It was found that the imprinted hydrogel had higher affinity to template molecules after modification. Investigations of grafting ratio influence on specific rebinding and competitive properties indicated that grafted side chains were beneficial to the selective recognition of protein. (C) 2014 Elsevier B.V. All rights reserved.