OXYTOCIN IN THE AMYGDALA FACILITATES MATERNAL AGGRESSION
OXYTOCIN IN THE AMYGDALA FACILITATES MATERNAL AGGRESSION
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DOI：
10.1111/j.1749-6632.1992.tb34382.x
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发表时间：
1992-06-12
期刊：
影响因子：
--
通讯作者：
INSEL, TR
中科院分区：
文献类型：
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作者：
FERRIS, CF;FOOTE, KB;INSEL, TR
催产素在主要位于下丘脑视上核和室旁核的大细胞神经元中合成。这种肽既可以作为从经典的下丘脑-神经垂体系统释放的神经激素来影响乳汁分泌，也可以作为从中枢神经系统中的神经末梢释放的神经递质来影响母体行为。在后一种情况下，据报道，脑室注射催产素可缩短未经生育、切除卵巢、雌激素治疗的大鼠的母性行为。相反地，催产素受体拮抗剂或抗血清的使用延迟了雌激素诱导的母性行为的发生。催产素可能起作用以诱导母性行为的中枢作用部位尚不清楚。催产素免疫反应性纤维的存在下，在杏仁核'colocalized与催产素绑定'表明，这个边缘网站可能参与催产素介导的motherapeutic behavior.Female金黄色仓鼠（n= 12），麻醉与戊巴比妥，并配备了双边26-gauge引导插管立体定向，旨在中央核的杏仁核。恢复后，对动物进行饲养，随后在妊娠期间将其圈养在单独的笼中。分娩后5天，在6天内每隔一天给动物注射（1）催产素（2 ng/200 nl）、（2）催产素受体拮抗剂（200 ng/200 nl）或（3）0.9%NaCl媒介物。母亲们被从笼子里拿出来注射。在它们离开期间，将幼仔从窝中取出并放置在饲养笼的另一侧。食物通常堆放在靠近巢穴的地方，现在散落在笼子里。将母亲放回笼中，并检查幼仔取回、食物取回、与幼仔的接触时间、幼仔梳理和护理。在这10分钟的测试期后，将一只成年雄性仓鼠放入笼中;并在5分钟内记录母亲的咬伤潜伏期、咬伤总数和与入侵者的总接触时间。这些处理之间在与幼仔护理相关的母体行为方面无显著差异。然而，有一个显着的治疗效果，对母亲的侵略方面的叮咬总数，总接触时间与入侵者。注射催产素的动物表现出显着更多的叮咬和接触时间的入侵者比注射催产素拮抗剂或生理盐水车辆。这些数据支持催产素可能在杏仁复合体中起作用以促进母亲的攻击性的观点。
Oxytocin is synthesized in magnocellular neurons primarily localized to the supraoptic nucleus and paraventricular nucleus of the hypothalamus.’This peptide can function both as a neurohormone released from the classical hypothalamo-neurohypophysial system to affect milk letdown and as a neurotransmitter released from nerve endings in the central nervous system to affect maternal behavior. In the latter case, it has been reported that intracerebroventricular injection of oxytocin shortens the onset of maternal behavior in nulliparous, ovariectomized, estrogen-treated rats.* Conversely, administration of oxytocin receptor antagonist or antiserum delays the onset of estrogen-induced maternal beha~ ior.~.~ The central sites of action at which oxytocin might function to induce maternal behavior are unclear. The presence of oxytocin-immunoreactive fibers in the amygdala’colocalized with oxytocin binding’suggest that this limbic site may be involved in oxytocin-mediated maternal behavior.Female golden hamsters (n= 12), were anesthetized with pentobarbital and fitted with bilateral 26-gauge guide cannulae stereotaxically aimed at the central nucleus of the amygdala. Following recovery, animals were bred and subsequently housed in individual cages for the duration of their pregnancies. Five days after giving birth, animals were injected every other day over six days with either (1) oxytocin (2 ng/200 nl),(2) oxytocin receptor antagonist (200 ng/200 nl), or (3) 0.9% NaCl vehicle. The mothers were removed from their home cages and injected. During their absence, the pups were removed from the nest and placed on the opposite side of the home cage. The food, which was normally piled in a location immediately adjacent to the nest, was scattered around the home cage. The mother was placed back into the cage and examined for pup retrieval, food retrieval, contact time with pups, pup grooming, and nursing. Following this 10-min test period, an adult male hamster was placed in the cage; and the mother’s latency to bite, total number of bites, and total contact time with intruder was recorded over five minutes. There were no significant differences between any of these treatments on maternal behavior associated with care of pups. However, there was a significant treatment effect on maternal aggression with respect to the total number of bites, and total contact time with the intruder. Animals injected with oxytocin displayed significantly more bites and contact time with the intruder than when injected with oxytocin antagonist or saline vehicle. These data support the notion that oxytocin may function in the amygdaloid complex to facilitate maternal aggression.