Preparation of an 18F-Labeled Hydrocyanine Dye as a Multimodal Probe for Reactive Oxygen Species
Preparation of an 18F-Labeled Hydrocyanine Dye as a Multimodal Probe for Reactive Oxygen Species
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DOI：
10.1002/chem.201604473
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发表时间：
2017-01-05
影响因子：
4.3
通讯作者：
Valliant, John F.
中科院分区：
文献类型：
--
作者：
Al-Karmi, Salma;Albu, Silvia A.;Valliant, John F.
氢菁染料是一种灵敏的“开启”型光学探针，可以检测活性氧（ROS）。我们已经开发了一种方法来制备F-18标记的氢菁染料作为多模式PET和光学“开启”探针。一种市售的近红外（NIR）染料改性与氟化辅基，不改变其活性氧传感性能的超氧化物和羟基自由基的存在下。18F-标记的类似物使用单步末端测序程序产生。正电子发射断层扫描（PET）成像和定量体内生物分布研究表明，这种新的探针有显着不同的药代动力学相比，氧化花菁类似物。化学报告，使使用定量和动态PET成像的体内研究的氢菁染料作为ROS探针。
Hydrocyanine dyes are sensitive "turn-on" type optical probes that can detect reactive oxygen species (ROS). We have developed a method to prepare an F-18-labeled hydrocyanine dye as a multi-modal PET and optical "turn-on" probe. A commercially available near infrared (NIR) dye was modified with a fluorinated prosthetic group that did not alter its ROS sensing properties in the presence of superoxide and hydroxyl radicals. The 18F-labeled analogue was produced using a single-step terminal fluorination procedure. Positron emission tomography (PET) imaging and quantitative in vivo biodistribution studies indicated this novel probe had remarkably different pharmacokinetics compared to the oxidized cyanine analogue. The chemistry reported enables the use of quantitative and dynamic PET imaging for the in vivo study of hydrocyanine dyes as ROS probes.