Focused ultrasound-mediated drug delivery from microbubbles reduces drug dose necessary for therapeutic effect on neointima formation--brief report.
Focused ultrasound-mediated drug delivery from microbubbles reduces drug dose necessary for therapeutic effect on neointima formation--brief report.
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DOI：
10.1161/atvbaha.111.238170
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Wamhoff BR
中科院分区：
文献类型：
--
作者：
Phillips LC;Dhanaliwala AH;Klibanov AL;Hossack JA;Wamhoff BR
我们假设：1)在大鼠颈动脉球囊损伤模型中，雷帕霉素负载微泡(RMBS)的靶向超声传递可以减少体内新生内膜的形成，以及2)双模超声的加入减少了减少新生内膜形成所需的药物总量。球囊损伤大鼠颈总动脉以诱导新生内膜形成。大剂量或小剂量RMBS静脉注射，在损伤部位超声破裂。与未处理的损伤动脉相比，108RMBS组新生内膜形成分别减少0%和35.9%，109RMBS+和不加超声处理组分别减少28.7%和34.9%。在没有超声的情况下，需要10倍浓度的RMBS才能减少新生内膜形成至少28%，而108 RMBS结合超声就足以达到同样的治疗效果，这表明这项技术可能有希望用于局部有效的药物治疗。
We hypothesized that 1) neointimal formation in a rat carotid balloon injury model could be reduced in vivo following targeted ultrasound delivery of rapamycin-loaded microbubbles (RMBs), and 2) the addition of dual mode ultrasound decreases the total amount of drug needed to reduce neointima formation. Balloon injury was performed in rat carotids to induce neointima formation. High or low doses of RMBs were injected I.V. and ruptured at the site of injury with ultrasound. Compared to non-treated injured arteries, neointima formation was reduced by 0% and 35.9% with 108 RMBs, and by 28.7% and 34.9% in arteries treated with 109 RMBs with and without ultrasound respectively. Without ultrasound, 10-fold higher concentrations of RMBs were needed to reduce neointima formation by at least 28%, whereas 108 RMBs combined with ultrasound were sufficient to achieve the same therapeutic effect demonstrating that this technology may have promise for localized potent drug therapy.