Requirement of nerve growth factor for development of substance P-containing sensory neurones
Requirement of nerve growth factor for development of substance P-containing sensory neurones
复制标题
含 P 物质的感觉神经元发育需要神经生长因子
作者：
U. Otten;M. Goedert;N. Mayer;F. Lembeck
已知蛋白质神经生长因子（NGF）对于肾上腺素能神经元的成熟和维持1以及对于胚胎生命的关键阶段期间感觉神经元的发育是必不可少的2 -4。迄今为止，由于缺乏这些神经元的生化标记物，对NGF在感觉神经元发育中的生理重要性的研究受到阻碍。十一肽P物质（SP）存在于感觉神经元中的证据表明，它可能是这样一个标志物。SP在背根神经节（DRG）7中合成，并运输到位于脊髓背角和皮肤中的C纤维的末端8。它的释放可以从感觉神经纤维的中央9，10和外周末梢11证明，这些感觉神经纤维似乎在疼痛感知中起重要作用12，13。我们研究了神经生长因子和纯化的抗神经生长因子抗体对大鼠背根神经节和它们各自的靶器官，即脊髓和皮肤中SP含量的影响。包括对交感神经节的影响，以控制NGF及其抗体的有效性。我们在这里报告，神经生长因子导致增加SP在脊神经节，如以前所示Kessler和Black 14。然而，与这些作者相反，我们描述了抗神经生长因子抗体的管理产生显着减少的SP含量在感觉神经元和各自的神经末梢。
The protein nerve growth factor (NGF) is known to be essential for the maturation and maintenance of adrenergic neurones1 and for the development of sensory neurones during critical stages of embryonic life2–4. The investigation of the physiological importance of NGF for the development of sensory neurones has been hampered so far by the lack of biochemical marker substances for these neurones. The demonstration that the undecapeptide substance P(SP) is present in sensory neurones5,6 suggests that it might be such a marker. SP is synthesized in dorsal root ganglia (DRG)7 and transported to the terminals of C-fibres located in the dorsal horn of the spinal cord and in the skin8. Its release can be demonstrated from the central9,10 and peripheral endings11 of sensory nerve fibres which seem to have an important role in pain perception12,13. We have investigated the effects of NGF and of purified anti-NGF antibodies on the content of SP in rat DRG and in their respective target organs, namely the spinal cord and the skin. The effects on sympathetic ganglia were included in order to control the effectiveness of both NGF and its antibody. We report here that NGF leads to an increase in SP in spinal ganglia, as previously shown by Kessler and Black14. However, in contrast to these authors, we describe that the administration of anti-NGF antibodies produces a marked reduction of the SP content in sensory neurones and in their respective nerve terminals.