Quantitative investigation of factors relevant to the T cell spot test for tuberculosis infection in active tuberculosis
Quantitative investigation of factors relevant to the T cell spot test for tuberculosis infection in active tuberculosis
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DOI：
10.1186/s12879-019-4310-y
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发表时间：
2019-07-29
影响因子：
3.7
通讯作者：
Dong, Xuemin
中科院分区：
文献类型：
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作者：
Li, Kui;Yang, Caiyong;Dong, Xuemin
以往的定性研究表明，结核感染T细胞斑点试验（T- spot . tb）的假阴性率与结核病患者的许多危险因素有关。然而，缺乏更精确的定量研究。本研究的目的是探讨影响活动性肺结核患者定量斑点形成细胞（sfc）早期分泌抗原靶蛋白6kDa （ESAT-6）或培养滤液蛋白10kDa （CFP-10）的因素。方法回顾性分析符合入选标准的360例患者资料。以sfc至ESAT-6或CFP-10水平为因变量，对单变量分析中具有统计学意义的变量进行最优标度回归分析。ESAT-6和CFP-10的结合(即T-SPOT。TB)采用精确logistic回归模型进行分析。结果sfc与ESAT-6的回归模型差异有统计学意义（P < 0.05）
BackgroundPrevious qualitative studies suggested that the false negative rate of the T cell spot test for tuberculosis infection (T-SPOT.TB) is associated with many risk factors in tuberculosis patients. However, more precise quantitative studies are lacking. The purpose of this study was to investigate the factors affecting quantified spot-forming cells (SFCs) to early secreted antigenic target 6kDa (ESAT-6) or culture filtrate protein 10kDa (CFP-10) in patients with active tuberculosis.MethodsWe retrospectively analyzed the data of 360 patients who met the inclusion criteria. Using the SFCs to ESAT-6 or CFP-10 levels as dependent variables, variables with statistical significance in the univariate analysis were subjected to optimal scaling regression analysis. The combination of ESAT-6 and CFP-10 (i.e., T-SPOT.TB) was analyzed by the exact logistic regression model.ResultsThe results showed that the SFCs to ESAT-6 regression model had statistical significance (P