Plasmacytoid Dendritic Cells Delineate Immunogenicity of Influenza Vaccine Subtypes
Plasmacytoid Dendritic Cells Delineate Immunogenicity of Influenza Vaccine Subtypes
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DOI：
10.1126/scitranslmed.3000759
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发表时间：
2010-03-31
影响因子：
17.1
通讯作者：
Ishii, Ken J.
中科院分区：
文献类型：
--
作者：
Koyama, Shohei;Aoshi, Taiki;Ishii, Ken J.
各种不同的疫苗类型可用于H1N1甲型流感病毒感染;然而，它们的免疫作用机制仍不清楚。在这里，我们表明，浆细胞样树突状细胞（pDC）和I型干扰素（IFN）介导的信号描绘的免疫原性减毒活病毒，灭活全病毒（WV），裂解病毒疫苗。虽然Toll样受体7作为活病毒和WV疫苗免疫原性的佐剂受体，但疫苗免疫原性对pDC产生I型IFN的要求仅限于WV。通常用于人类的裂解疫苗未能免疫幼稚小鼠，但pDC活化佐剂可以恢复免疫原性。然而，在成年人的血液中，单独的裂解疫苗可以回忆记忆T细胞反应，强调了这种佐剂途径对初次接种而不是二次接种的重要性。
A variety of different vaccine types are available for H1N1 influenza A virus infections; however, their immunological mechanisms of action remain unclear. Here, we show that plasmacytoid dendritic cells (pDCs) and type I interferon (IFN)-mediated signaling delineate the immunogenicity of live attenuated virus, inactivated whole-virus (WV), and split virus vaccines. Although Toll-like receptor 7 acted as the adjuvant receptor for the immunogenicity of both live virus and WV vaccines, the requirement for type I IFN production by pDCs for the immunogenicity of the vaccines was restricted to WV. A split vaccine commonly used in humans failed to immunize naive mice, but a pDC-activating adjuvant could restore immunogenicity. In blood from human adults, however, split vaccine alone could recall memory T cell responses, underscoring the importance of this adjuvant pathway for primary, but not secondary, vaccination.