Gonadotropin-induced murine oocyte maturation in vivo is not associated with decreased cyclic adenosine monophosphate in the oocyte-cumulus cell complex.
Gonadotropin-induced murine oocyte maturation in vivo is not associated with decreased cyclic adenosine monophosphate in the oocyte-cumulus cell complex.
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促性腺激素诱导的体内小鼠卵母细胞成熟与卵母细胞-卵丘细胞复合物中环磷酸腺苷的减少无关。
DOI：
10.1002/mrd.1120200203
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Downs,SM
中科院分区：
文献类型：
--
作者：
Eppig,JJ;Downs,SM
小鼠体内诱导卵母细胞成熟后不同时间内完整卵母细胞-卵丘细胞复合物环磷酸腺苷（cAMP）含量与卵母细胞参与生发囊泡破裂（GVB）和卵母细胞与卵丘细胞之间代谢偶联的时间相关。79%的卵母细胞在注射人绒毛膜促性腺激素（hCG） 2小时后发生GVB或承诺发生GVB。在这种情况下，卵丘细胞与卵母细胞之间的偶联没有减少，而卵母细胞-卵丘细胞复合体中的cAMP水平却增加了。维持哺乳动物卵母细胞内cAMP的阈值水平似乎对维持减数分裂停滞至关重要，但本文提供的数据表明，小鼠卵母细胞成熟是由体内非闭锁卵泡中的促性腺激素通过某种机制诱导的，而不是通过降低完整卵母细胞-卵囊细胞复合物中cAMP含量的机制。
The cyclic adenosine monophosphate (cAMP) content of intact oocyte‐cumulus cell complexes at various times after the induction of oocyte maturation in mice in vivo was correlated with the time of commitment by the oocytes to undergo germinal vesicle breakdown (GVB) and metabolic coupling between the oocyte and cumulus cells. Seventy‐nine percent of the oocytes either underwent GVB or were committed to do so by 2 h after injection of human chorionic gonadotropin (hCG). This occurred without a decrease in the coupling between cumulus cells and the oocyte and with increasing cAMP levels in the oocyte‐cumulus cell complex. Maintenance of threshold levels of cAMP within mammalian oocytes appears essential for the maintenance of meiotic arrest, but data presented here suggest that oocyte maturation in mice is induced by gonadotropins in nonatretic follicles in vivo by some mechanism other than one which decreases the cAMP content of the intact oocyte‐cumulus cell complex.