Differential release of corticotropin-releasing hormone (CRH) in the amygdala during different types of stressors
Differential release of corticotropin-releasing hormone (CRH) in the amygdala during different types of stressors
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DOI：
10.1016/s0006-8993(02)02972-4
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发表时间：
2002-09-13
期刊：
影响因子：
2.9
通讯作者：
Wilson, MA
中科院分区：
文献类型：
--
作者：
Hand, GA;Hewitt, CB;Wilson, MA
本研究旨在探讨急性跑台运动和束缚应激对大鼠杏仁核促肾上腺皮质激素释放激素（CRH）释放的影响是否存在差异。细胞外CRH免疫反应性（CRH-IR）测定暴露于失活的跑步机（TC），在IIT跑步机运行到耗尽（RUN），和IIT约束（RES）期间收集的杏仁核（CeA）的微透析液。细胞外CRH-IR增加，从控制水平在第一个20分钟期间的TC，RUN和RES，与最大的增加在RES。在第二个20分钟期间，只有RES保持高于控制值的水平。在RES和RUN的第三个20 min期间，CRH释放高于对照。第二个实验由笼对照（CC）、TC、RUN或RES四组组成。60分钟处理后，立即取出大脑并收集躯干血液用于组织CRH-IR和血浆皮质酮分析。虽然杏仁核组织CRH-IR在CC、TC和RUN大鼠中没有差异，但这些组的水平显著低于RES动物。下丘脑组织CRH-IR在CC组和TC组之间无差异，但RES组和RUN组的水平显著高于两个对照组。组织分析结果表明，RES可引起杏仁核和下丘脑组织CRH-IR增加，而RUN后仅下丘脑CRH-IR增加。此外，在整个RES期间，当大鼠被放置在跑步机上时，以及当动物接近体力耗尽时，CeA中的细胞外CRH释放增加。在放置在跑步机上和剧烈运动之间的跑步期间没有观察到增加。总的来说，这些数据表明，杏仁核CRH的释放在跑步机运行和约束不同的调节。(C)2002 Elsevier Science B. V.保留所有权利。
The purpose of this study was to determine if differences exist between the effects of acute treadmill running and restraint stress on corticotropin-releasing hormone (CRH) release within the amygdala of rats. Extracellular CRH immunoreactivity (CRH-IR) was measured in microdialysate collected from the central nucleus of the amygdala (CeA) during exposure to an inactivated treadmill (TC), during I It treadmill running to exhaustion (RUN), and I It restraint (RES). Extracellular CRH-IR increased from control levels during the first 20-min period for TC, RUN, and RES, with the largest increase during RES. During the second 20-min period, only RES maintained levels higher than control values. CRH release was higher than control during the third 20-min period of RES and RUN. A second experiment consisted of four groups of either cage controls (CC), TC, RUN, or RES. Immediately following the 60-min treatment, brains were removed and trunk blood collected for analysis of tissue CRH-IR and plasma corticosterone. While amygdala tissue CRH-IR was not different in the CC, TC and RUNT rats, these groups had significantly lower levels than the RES animals. Hypothalamic tissue CRH-IR was not different between the CC and TC rats, but the levels were significantly higher in the RES and RUN rats than in the two control groups. Plasma corticosterone levels were elevated only in RES and RUN rats, Results from tissue analysis indicate that increased tissue CRH-IR in the amygdala and hypothalamus can be elicited by RES, while only the hypothalamus shows an increase following RUN. Further, extracellular CRH release in the CeA is increased throughout the period of RES, when rats are placed on the treadmill, and when the animals are approaching physical exhaustion. No increase is observed during the running period between placement on the treadmill and intense exertion. Overall, the data suggest that amygdala CRH release is regulated differently during treadmill running and restraint. (C) 2002 Elsevier Science B.V. All rights reserved.