Two domains at the origin are required for replication and maintenance of broad-host-range plasmid R1162.
Two domains at the origin are required for replication and maintenance of broad-host-range plasmid R1162.
复制标题
复制和维持广宿主范围质粒 R1162 需要在起点有两个结构域。
DOI：
10.1128/jb.169.12.5870-5872.1987
复制
发表时间：
1987
影响因子：
3.2
通讯作者：
Meyer,RJ
中科院分区：
文献类型：
--
作者：
Kim,YJ;Lin,LS;Meyer,RJ
广宿主质粒R1162的复制起始处的两个结构域在顺式结构中是必需的，以便在大肠杆菌中保持质粒，并在细胞提取液中复制质粒DNA。增加结构域之间的距离会减少体外复制，而不会显著改变质粒DNA的含量或体内的稳定性。
Two domains at the replicative origin of broad-host-range plasmid R1162 are required in cis for plasmid maintenance in Escherichia coli and for plasmid DNA replication in cell extracts. Increasing the distance between the domains reduces replication in vitro, without substantially changing plasmid DNA content or stability in vivo.