The presence of two low molecular mass proteins immunologically related to 14 kilodalton serum amyloid A in the lipoprotein fraction and their decreased serum concentrations in calves with experimentally induced pneumonia.
The presence of two low molecular mass proteins immunologically related to 14 kilodalton serum amyloid A in the lipoprotein fraction and their decreased serum concentrations in calves with experimentally induced pneumonia.
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脂蛋白部分中存在两种与 14 kD 血清淀粉样蛋白 A 免疫学相关的低分子量蛋白，并且在实验诱发肺炎的小牛中，它们的血清浓度降低。
DOI：
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发表时间：
1998
影响因子：
1.2
通讯作者：
Y. Adachi
中科院分区：
文献类型：
--
作者：
Midori Yamamoto;N. Katoh;Y. Adachi
从牛血清中纯化了一个14千道尔顿（kDa）的血清淀粉样蛋白A （apoSAA）蛋白。兔抗血清对14 kDa apoSAA除能识别14 kDa蛋白外，还能识别7.5-9.0 kDa蛋白和分子质量小于6.5 kDa的蛋白（< 6.5 kDa蛋白）。结果显示，免疫印迹分析显示，纯化过程早期检测到的两种蛋白质在纯化的14 kDa apoSAA片段中未发现，排除了这两种蛋白质是污染物的可能性。与14 kDa apoSAA一样，7.5-9.0 kDa的蛋白位于高密度脂蛋白部分，而< 6.5 kDa的蛋白位于低密度脂蛋白部分。在接种溶血巴氏杆菌引起的肺炎犊牛血清中，14 kDa apoSAA蛋白的浓度升高，而7.5-9.0 kDa和< 6.5 kDa的apoSAA蛋白的浓度则相反降低。时间过程研究表明，14 kDa apoSAA蛋白浓度的升高与< 6.5 kDa apoSAA蛋白浓度的降低几乎同时发生。这些结果表明，14 kDa apoSAA蛋白和免疫相关的7.5-9.0 kDa蛋白和< 6.5 kDa蛋白分别作为急性期阳性和阴性反应物，并且这三种蛋白的浓度在急性期血浆中是一致调节的。
A 14 kilodalton (kDa) serum amyloid A (apoSAA) protein was purified from cow serum. Rabbit antiserum to the 14 kDa apoSAA recognized, in addition to the 14 kDa protein, a 7.5-9.0 kDa protein and a protein having a molecular mass of less than 6.5 kDa (< 6.5 kDa protein). The possibility that the two proteins were contaminants was excluded by results showing that the two proteins detected in early stages of purification procedures were not found in the purified 14 kDa apoSAA fraction, as revealed by immunoblot analysis. As in the 14 kDa apoSAA, the 7.5-9.0 kDa protein was localized in the high-density lipoprotein fraction, while the < 6.5 kDa protein was in the low-density lipoprotein fraction. In calves with pneumonia induced by inoculation to the lungs of Pasteurella haemolitica, the serum concentration of the 14 kDa apoSAA was increased, whereas those of the 7.5-9.0 kDa and the < 6.5 kDa proteins were conversely decreased. The time-course study indicated that the increase in concentration of the 14 kDa apoSAA and decrease in that of the < 6.5 kDa protein occurred almost simultaneously. These results suggest that the 14 kDa apoSAA and the immunologically related 7.5-9.0 kDa and < 6.5 kDa proteins act as positive and negative acute phase reactants, respectively, and also that concentrations of the three proteins are regulated in concert in acute phase plasma.
非人灵长类动物（黑长尾猴）高密度脂蛋白和乳糜微粒中血清淀粉样蛋白 A (SSA) 的代谢。
DOI：
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发表时间：
1983
期刊：
The American journal of pathology
影响因子：
--
作者：
Parks,JS;Rudel,LL
通讯作者：
Rudel,LL
重组人血清淀粉样蛋白 A (apoSAAp) 结合胆固醇并调节胆固醇通量。
影响因子：
6.5
作者：
Liang,JS;Sipe,JD
通讯作者：
Sipe,JD