Application of microbial chip for amperometric detection of metabolic alteration in bacteria
Application of microbial chip for amperometric detection of metabolic alteration in bacteria
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DOI：
10.1016/j.snb.2004.10.050
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发表时间：
2005-07-22
影响因子：
8.4
通讯作者：
Matsue, T
中科院分区：
文献类型：
--
作者：
Nagamine, K;Kaya, T;Matsue, T
利用3.5nL体积的胶原胶基质包埋细菌细胞的微生物芯片，研究了细菌对高渗透胁迫的反应。扫描电化学显微镜(SECM)用于监测Fe(CN)(6)(4-)的产生，它反映了细菌细胞膜内呼吸链上的电子流动。在目前的研究中，我们发现在高浓度盐的存在下，金黄色葡萄球菌中的铁氰化物还原活性被增强。这一结果表明，渗透胁迫引起细胞膜结构或膜相关蛋白谱的改变，从而提高细胞膜对亲水性介质的通透性。金黄色葡萄球菌的低电流响应性不仅可以通过以下方法得到改善：(1)使用疏水介质，如取代Fe(CN)(6)(3-)的苯醌衍生物；(2)在亲水性介质存在的情况下，使金黄色葡萄球菌细胞暴露在高渗透胁迫下。(C)2004爱思唯尔B.V.保留所有权利。
The microbial chip of 3.5 nL-volume of collagen gel matrixes entrapping the bacterial cells in living state has been applied to investigate the bacterial response to high osmotic stress. Scanning electrochemical microscopy (SECM) was used to monitor the Fe(CN)(6)(4-) production that reflects the electron flow at respiratory chain located within the cytoplasmic membrane of bacteria. In the present study, we have found that the ferricyanide reduction activity in the Staphylococcus aureus, osmotolerant bacteria, is enhanced in the presence of highly concentrated salts. This result suggests that the osmotic stress causes the change in membrane structure or membrane-associated protein profile so as to enhance the cell membrane permeability to hydrophilic mediator. The low current responsibility of S. aureus can be improved not only by (1) using hydrophobic mediator, such as quinone derivatives instead of Fe(CN)(6)(3-) but also (2) exposing S. aureus cells to high osmotic stress in the presence of hydrophilic mediator. (c) 2004 Elsevier B.V. All rights reserved.