Antiviral effect of Saccharomyces cerevisiae β-glucan to swine influenza virus by increased production of interferon-γ and nitric oxide
Antiviral effect of Saccharomyces cerevisiae β-glucan to swine influenza virus by increased production of interferon-γ and nitric oxide
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DOI：
10.1111/j.1439-0450.2004.00732.x
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发表时间：
2004-03-01
期刊：
影响因子：
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通讯作者：
Chae, C
中科院分区：
文献类型：
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作者：
Jung, K;Ha, Y;Chae, C
本实验旨在探讨酿酒酵母β-葡聚糖对猪流感病毒(SIV)所致肺炎的潜在抗病毒作用。将40头5日龄初乳剥夺仔猪随机分为4组，每组10头。第1组和第2组在SIN感染前连续3d口服酿酒酵母P-葡聚糖(50 mg/天/头，EN-Bio Technology Co.，Ltd.)，第3组和第4组单独给予培养液/稀释液。第1组和第3组鼻腔接种含2×106组织培养感染量50%(TCID50)/ml SIV的组织培养液3ml，第2组和第4组以相同方式暴露于未感染的细胞培养上清中。注射β-葡聚糖前，SIV感染(第I组)引起的肺损伤明显重于注射β-葡聚糖(第3组)(P<；0.05)。与注射P-葡聚糖和感染SIV的猪(组3)相比，实验中仅感染SIN的猪(组1)在接种后5、7和10天的肺中检出的SIV核酸显著增加(P<；0.05)。感染SIV的猪在注射β-葡聚糖前，肺泡灌洗液中的干扰素-γ和一氧化氮(NO)浓度在给药后7dpi和10dpi、5、7dpi和10dpi时显著高于其他各组(P<0.05)。酿酒酵母β-葡聚糖可降低SIV感染猪的肺部病变评分和病毒复制率。这些发现支持了β-葡聚糖作为流感病毒感染的预防/治疗药物的潜在应用。
The aim of these experiments was to investigate the potential antiviral effect of Saccharomyces cerevisiae beta-glucan on the pneumonia induced by swine influenza virus (SIV). Forty colostrum-deprived 5-day-old piglets were randomly divided into four groups of 10. The 20 pigs in groups I and 2 were administered Saccharomyces cerevisiae P-glucan orally (50 mg/day/pig; En-Bio Technology Co., Ltd) for 3 days before SIN infection and those in groups 3 and 4 were given culture medium/diluent alone. Groups I and 3 were inoculated intranasally with 3 ml of tissue culture fluid containing 2 x 106 tissue culture infective doses 50% (TCID50)/ml Of SIV and those in groups 2 and 4 were exposed in the same manner to uninfected cell culture supernatant. The microscopic lung lesions induced by SIV infection (group I pigs) were significantly more severe than those induced by infection in animals pre-administered beta-glucan (group 3) (P < 0.05). Significantly more SIV nucleic acid was detected in the lungs of pigs experimentally infected with SIN only (group 1) at 5, 7 and 10 days post-inoculation (dpi) compared with lungs from pigs pre-administered P-glucan and infected with SIV (group 3) (P < 0.05). The concentrations of interferon-gamma (IFN-gamma) and nitric oxide (NO) in bronchoalveolar lavage fluid from pigs pre-administered beta-glucan and infected with SIV (group 3) were significantly higher than for any other group at 7 and 10 dpi for IFN-gamma, and at 5, 7 and 10 dpi for NO (P < 0.05). Saccharomyces cerevisiae beta-glucan reduced the pulmonary lesion score and viral replication rate in SIV-infected pigs. These findings support the potential application of beta-glucan as prophylactic/treatment agent in influenza virus infection.