Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity.
Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity.
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DOI：
10.1038/s41467-017-01447-x
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发表时间：
2017-11-08
影响因子：
16.6
通讯作者：
Gazit E
中科院分区：
文献类型：
--
作者：
Schnaider L;Brahmachari S;Schmidt NW;Mensa B;Shaham-Niv S;Bychenko D;Adler-Abramovich L;Shimon LJW;Kolusheva S;DeGrado WF;Gazit E
基于肽的超分子组装体是一类具有重要生物医学应用前景的纳米材料，特别是在药物递送和组织再生方面。然而，这些组件的内在抗菌能力在很大程度上被忽视了。最近鉴定的抗菌肽和自组装肽共有的共同特征为抗菌剂的开发提供了一个范式转变。在这里，我们提出了自组装的二苯丙氨酸的抗菌活性，这是抗菌超分子聚合物的最小模型。二苯丙氨酸纳米组装体完全抑制细菌生长，触发应激反应调节子的上调，诱导细菌形态的实质性破坏，并引起膜渗透和去极化。我们证明了这些膜相互作用的特异性和抗菌材料的发展，通过整合的肽组件到组织支架。这项研究为自组装和抗菌活性之间相互作用的意义提供了重要的见解，并建立了抗菌剂和材料开发的创新设计原则。基于肽的超分子组装体是一类具有重要生物医学应用前景的纳米材料，但其抗菌性能却被忽视。在这里，作者展示了自组装的二苯丙氨酸的抗菌活性，这是抗菌超分子聚合物的最小模型。
Peptide-based supramolecular assemblies are a promising class of nanomaterials with important biomedical applications, specifically in drug delivery and tissue regeneration. However, the intrinsic antibacterial capabilities of these assemblies have been largely overlooked. The recent identification of common characteristics shared by antibacterial and self-assembling peptides provides a paradigm shift towards development of antibacterial agents. Here we present the antibacterial activity of self-assembled diphenylalanine, which emerges as the minimal model for antibacterial supramolecular polymers. The diphenylalanine nano-assemblies completely inhibit bacterial growth, trigger upregulation of stress-response regulons, induce substantial disruption to bacterial morphology, and cause membrane permeation and depolarization. We demonstrate the specificity of these membrane interactions and the development of antibacterial materials by integration of the peptide assemblies into tissue scaffolds. This study provides important insights into the significance of the interplay between self-assembly and antimicrobial activity and establishes innovative design principles toward the development of antimicrobial agents and materials. Peptide-based supramolecular assemblies are a promising class of nanomaterials with important biomedical applications, but their antibacterial properties can be overlooked. Here the authors show the antibacterial activity of self-assembled diphenylalanine, which emerges as the minimal model for antibacterial supramolecular polymers.
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影响因子：
3.7
作者：
Caillon L;Killian JA;Lequin O;Khemtémourian L
通讯作者：
Khemtémourian L
影响因子：
5.4
作者：
Beatman, Erica L.;Massey, Aaron;Beckham, J. David
通讯作者：
Beckham, J. David
DOI：
10.1073/pnas.97.16.8856
发表时间：
2000-08-01
影响因子：
11.1
作者：
Hancock, REW;Scott, MG
通讯作者：
Scott, MG
影响因子：
5.4
作者：
Auvynet, Constance;El Amri, Chahrazade;Rosenstein, Yvonne
通讯作者：
Rosenstein, Yvonne
影响因子：
3.4
作者：
Jang, Hyunbum;Arce, Fernando Teran;Lal, Ratnesh
通讯作者：
Lal, Ratnesh