Magnetic/Fluorescent Barcodes Based on Cadmium-Free Near-Infrared-Emitting Quantum Dots for Multiplexed Detection
Magnetic/Fluorescent Barcodes Based on Cadmium-Free Near-Infrared-Emitting Quantum Dots for Multiplexed Detection
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基于无镉近红外发射量子点的磁性/荧光条形码，用于多重检测
DOI：
10.1002/adfm.201602900
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发表时间：
2016-11-08
影响因子：
19
通讯作者：
Li, Wanwan
中科院分区：
文献类型：
--
作者：
Leng, Yuankui;Wu, Weijie;Li, Wanwan
磁性/荧光条形码将量子点 (QD) 和超顺磁性纳米粒子结合在微米大小的宿主微球中，有望用于自动高通量多重生物检测应用和“护理点”生物检测。然而，在添加磁性纳米颗粒（MNP）后，由于MNP的吸收，QD的荧光强度急剧下降，因此，QD的编码能力变得更加有限。此外，条形码中最常用的量子点——镉基量子点的内在毒性对人类健康和环境具有重大风险。在这项工作中，为了减轻荧光猝灭和内在毒性，利用 Shirasu 多孔玻璃膜乳化技术，成功地将无镉的近红外发射 CuInS2/ZnS QD 和 Fe3O4 MNP 掺入聚（苯乙烯-马来酸酐）微球中。成功构建了“单波长”编码模型来指导宽发射光谱的近红外量子点的编码。然后，采用“单波长”编码与尺寸编码相结合，通过简单地改变量子点的波长和强度以及条形码微球的尺寸来产生不同的光学代码。由于近红外发射 QD 和 MNP 之间的能量传输大大减少，因此可以轻松创建 48 个条形码。由此产生的双功能条形码还与流式细胞仪结合使用，使用一束激光在一次测试中多重检测五种肿瘤标志物。基于这些条形码的检测比非磁性和传统 ELISA 检测更加灵敏。此外，验证实验还表明，基于双功能条形码的悬浮阵列在处理患者血清样本时具有良好的性能。因此，基于近红外发射 CuInS2/ZnS QD 的磁性/荧光条形码有望用于多重生物测定应用。
Magnetic/fluorescent barcodes, which combine quantum dots (QDs) and superparamagnetic nanoparticles in micrometer-sized host microspheres, are promising for automatic high-throughput multiplexed biodetection applications and "point of care" biodetection. However, the fluorescence intensity of QDs sharply decreases after addition of magnetic nanoparticles (MNPs) due to absorption by MNPs, and thus, the encoding capacity of QDs becomes more limited. Furthermore, the intrinsic toxicity of cadmium-based QDs, the most commonly used QD in barcodes, has significant risks to human health and the environment. In this work, to alleviate fluorescence quenching and intrinsic toxicity, cadmium-free NIR-emitting CuInS2/ZnS QDs and Fe3O4 MNPs are successfully incorporated into poly(styrene-co-maleic anhydride) microspheres by using the Shirasu porous glass membrane emulsification technique. A "single-wavelength" encoding model is successfully constructed to guide the encoding of NIR QDs with wide emission spectra. Then, a "single-wavelength" encoding combined with size encoding is used to produce different optical codes by simply changing the wavelength and the intensity of the QDs as well as the size of the barcode microspheres. 48 barcodes are easily created due to the greatly reduced energy transfer between the NIR-emitting QDs and MNPs. The resulting bifunctional barcodes are also combined with a flow cytometer using one laser for multiplexed detection of five tumor markers in one test. Assays based on these barcodes are significantly more sensitive than non-magnetic and traditional ELISA assays. Moreover, validating experiments also show good performance of the bifunctional barcodes-based suspension array when dealing with patient serum samples. Thus, magnetic/fluorescent barcodes based on NIR-emitting CuInS2/ZnS QDs are promising for multiplexed bioassay applications.