The United States Potato Genebank Holding of cv. Desiree is a Somatic Mutant of cv. Urgenta
The United States Potato Genebank Holding of cv. Desiree is a Somatic Mutant of cv. Urgenta
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DOI：
10.1007/s12230-022-09892-1
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发表时间：
2022-12-07
影响因子：
1.5
通讯作者：
Comai，Luca
中科院分区：
文献类型：
--
作者：
Amundson，Kirk R.;Henry，Isabelle M.;Comai，Luca
全基因组标记可以常规确认品种是否符合类型，如果不符合，则可以推断其身份。本研究的目的是确定先前描述的染色体易位 tr8-7 的基础，该染色体易位在四倍体马铃薯 (Solanum tuberosumL.) 品种 Desiree 的持有中明显具有多态性。通过分析来自北美和欧洲四倍体马铃薯种质的公开基因型数据，我们解决了美国马铃薯基因库持有的 Desiree 的长期错误身份，它实际上是其母本 cv. 的体细胞突变体。紧急。对多个持有物的比较表明，tr8-7 是至少 25 年前发生的体细胞突变，并在孤立的谱系中得以维持。来自其他机构的藏品缺少 tr8-7，并通过三重分析确认为 Desiree，这表明这种混淆影响了美国马铃薯种质库以及从那里接收 Desiree 的机构。面对不可避免的突变和人为错误，我们建议利用谱系和基因组信息验证马铃薯种质资源。为此，我们提供了分子标记来区分本研究中研究的品种。
Genome-wide markers enable routine confirmation of whether varieties are true-to-type, and when they are not, to infer their identity. The objective of this study was to determine the basis of a previously described chromosome translocation, tr8-7, which was apparently polymorphic among holdings of the tetraploid potato (Solanum tuberosumL.) cultivar Desiree. Through analysis of publicly available genotype data from North American and European tetraploid potato germplasm, we resolved a longstanding mistaken identity of the United States Potato Genebank holding of Desiree, which is actually a somatic mutant of its maternal parent, cv. Urgenta. Comparison of multiple holdings revealed that tr8-7 was a somatic mutation that occurred at least 25 years ago and was maintained in isolated lineages. Holdings from other institutions lacked tr8-7 and were confirmed as Desiree by trio analyses, suggesting that the mixup affected the United States Potato Genebank and institutions that received Desiree from there. In the face of inevitable mutations and human error, we recommend validation of potato germplasm collections with pedigree and genomic information. To that effect, we provide molecular markers to distinguish the varieties investigated in this study.