Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae
Overproduction of geraniol by enhanced precursor supply in Saccharomyces cerevisiae
复制标题
通过增强酿酒酵母前体供应来过量生产香叶醇
DOI：
10.1016/j.jbiotec.2013.10.017
复制
发表时间：
2013-12-01
影响因子：
4.1
通讯作者：
Zhou, Jingwen
中科院分区：
文献类型：
--
作者：
Liu, Jidong;Zhang, Weiping;Zhou, Jingwen
单萜香叶醇是一种从芳香植物中提取的化合物，具有广泛的用途。本研究通过引入香叶醇合成酶，在酿酒酵母中合成香叶醇。为了提高香叶醇的产量，S.对酿酒酵母进行遗传操作以增强香叶基二磷酸（用于香叶醇生物合成的底物）的供应。目的：鉴定并优化S.酿酒酵母使香叶醇产量提高到36.04 mg L-1。结果表明，IDI 1编码的异戊烯基二磷酸异构酶是S.而过量表达tRNA生物合成负调控因子MAF 1是提高S. cerevisiae中香叶醇产量的另一种有效方法。啤酒。(C)2013爱思唯尔有限公司版权所有。
Monoterpene geraniol, a compound obtained from aromatic plants, has wide applications. In this study, geraniol was synthesized in Saccharomyces cerevisiae through the introduction of geraniol synthase. To increase geraniol production, the mevalonate pathway in S. cerevisiae was genetically manipulated to enhance the supply of geranyl diphosphate, a substrate used for the biosynthesis of geraniol. Identification and optimization of the key regulatory points in the mevalonate pathway in S. cerevisiae increased geraniol production to 36.04 mg L-1. The results obtained revealed that the IDI1-encoded isopentenyl diphosphate isomerase is a rate-limiting enzyme in the biosynthesis of geraniol in S. cerevisiae, and overexpression of MAF1, a negative regulator in tRNA biosynthesis, is another effective method to increase geraniol production in S. cerevisiae. (C) 2013 Elsevier B.V. All rights reserved.