Application of AAV1 for Anterograde Transsynaptic Circuit Mapping and Input-Dependent Neuronal Cataloging.
Application of AAV1 for Anterograde Transsynaptic Circuit Mapping and Input-Dependent Neuronal Cataloging.
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DOI：
10.1002/cpz1.339
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Zhang LI
中科院分区：
文献类型：
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作者：
Zingg B;Dong HW;Tao HW;Zhang LI
通过突触传播的病毒为研究大脑中相互连接的回路提供了强有力的手段。在这里，我们描述了使用腺相关病毒，血清型1 （AAV1）作为在各种单向途径中实现稳健，顺行跨突触传播的工具。提出了在小鼠中进行颅内AAV1注射的方案，以及在计划实验，采购材料和优化方法方面的额外指导，以获得最成功的结果。按照本文提出的方法，研究人员将能够揭示给定AAV1注射部位下游突触后连接的神经元，并访问这些输入定义的细胞进行后续的绘图、记录和操作，以表征其解剖和功能特性。
Viruses that spread transsynaptically provide a powerful means for studying interconnected circuits in the brain. Here we describe the use of adeno-associated virus, serotype 1 (AAV1) as a tool for achieving robust, anterograde transsynaptic spread in a variety of unidirectional pathways. A protocol for performing intracranial AAV1 injections in mice is presented, along with additional guidance in planning experiments, sourcing materials, and optimizing the approach to achieve the most successful outcomes. Following the methods presented here, researchers will be able to reveal postsynaptically connected neurons downstream of a given AAV1 injection site, and access these input-defined cells for subsequent mapping, recording, and manipulation to characterize their anatomical and functional properties.