Intravenous omega-3, a technique to prevent an excessive innate immune response to cardiac surgery in a rodent gut ischemia model
Intravenous omega-3, a technique to prevent an excessive innate immune response to cardiac surgery in a rodent gut ischemia model
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DOI：
10.1016/j.jtcvs.2010.04.030
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发表时间：
2011-03-01
影响因子：
6
通讯作者：
Redmond, Mark J.
中科院分区：
文献类型：
--
作者：
Byrne, John;McGuinness, Jonathan;Redmond, Mark J.
目的：作为心脏手术炎症反应的一部分，组织的神经浸润是术后多器官功能障碍的主要介质之一。ω-3脂肪酸显著减弱内皮细胞炎症反应，包括中性粒细胞粘附分子的上调。临床上安全的形式的omega-3产生这种效果在vivo的疗效examined.Methods：大鼠肠道活体显微镜分析被用来可视化从微循环到肠道组织的中性粒细胞transmigration。炎症激活的形式是缺血30分钟和再灌注90分钟。骗徒，控制（0.9%生理盐水输注4小时）和omega-3结果：缺血再灌注导致中性粒细胞粘附内皮细胞增加4倍，（基线：4.3 ± 0.2 vs对照组：19.2 ± 3.5粘附中性粒细胞/100 μ m，P <0.01），静脉内ω-3抑制（7.8 ± 1.7粘附中性粒细胞/100 μ m，P <0.01）。欧米茄-3预处理还减少了再灌注后中性粒细胞向组织中的迁移（假手术组：6.3 +/- 0.8 vs对照组：13.2 +/- 1.4 vs欧米茄-3组：9.4 +/- 0.9中性粒细胞/视野，P = 0.037）。肠道组织中的嗜中性粒细胞释放的酶髓过氧化物酶的水平同样显着降低与ω-3预处理（假手术组：10.5 +/- 1.6 vs对照组：19.0 +/- 3.3 vs omega-3组：10.1 +/- 1.2 U/g，P = 0.03）。用相对安全的静脉注射omega-3预处理4小时，可以抑制中性粒细胞粘附和组织浸润，从而降低组织损伤酶髓过氧化物酶的水平。这提示了减少术后多器官功能障碍的可能策略。（《胸血管外科杂志》2011;141：803-7）
Objectives: Neutrophil infiltration of tissues as part of the inflammatory response to cardiac surgery is one of the major mediators of postoperative multiple-organ dysfunction. Omega-3 fatty acids markedly attenuate endothelial cell inflammatory responses, including upregulation of neutrophil adhesion molecules. The efficacy of a clinically safe form of omega-3 to produce this effect in vivo was examined.Methods: Rat gut intravital microscopic analysis was used to visualize neutrophil transmigration from the microcirculation into the tissues of the gut. Inflammatory activation was in the form of 30 minutes of ischemia and 90 minutes of reperfusion. Sham, control (0.9% saline infusion over 4 hours), and omega-3 (Omegaven [Fresenius Kabi, Bad Homburg, Germany] infusion over 4 hours) pretreatments were compared.Results: Ischemia-reperfusion resulted in a 4-fold increase in neutrophil adherence to the endothelium (baseline: 4.3 +/- 0.2 vs control group: 19.2 +/- 3.5 adherent neutrophils per 100 mu m, P < .01), which intravenous omega-3 suppressed (7.8 +/- 1.7 adherent neutrophils per 100 mu m, P < .01). Omega-3 pretreatment also reduced neutrophil transmigration into the tissues after reperfusion (sham group: 6.3 +/- 0.8 vs control group: 13.2 +/- 1.4 vs omega-3 group: 9.4 +/- 0.9 neutrophils per field, P = .037). Gut tissue levels of the neutrophil-released enzyme myeloperoxidase were similarly markedly reduced with omega-3 pretreatment (sham group: 10.5 +/- 1.6 vs control group: 19.0 +/- 3.3 vs omega-3 group: 10.1 +/- 1.2 U/g, P = .03).Conclusions: Four hours' pretreatment with a relatively safe form of intravenous omega-3 suppressed neutrophil adherence and tissue infiltration, resulting in lower levels of the tissue-damaging enzyme myeloperoxidase. This suggests a possible strategy for diminishing postoperative multiple-organ dysfunction. (J Thorac Cardiovasc Surg 2011;141:803-7)