No differences in efficacy between noncultured and cultured islets in reducing hyperglycemia in a nonvascularized islet graft model
No differences in efficacy between noncultured and cultured islets in reducing hyperglycemia in a nonvascularized islet graft model
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DOI：
10.1089/dia.2006.8.536
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发表时间：
2006-10-01
影响因子：
5.4
通讯作者：
King, Aileen
中科院分区：
文献类型：
--
作者：
Bohman, Sara;Andersson, Arne;King, Aileen
背景资料：虽然胰岛移植是一种很有前途的方法，以恢复正常的受体糖尿病，仍然需要大量的胰岛。已经表明，使用新鲜分离的胰岛可以通过更好的血管植入来改善移植结果。使用的微囊化技术，一个模型，其中血运重建是不可能的，我们调查的重要性，血运重建的移植outcome.Methods：无论是700或350个3天培养或noncultured封装胰岛腹膜内移植到同系小鼠四氧嘧啶诱导的糖尿病。此外，700个未封装的胰岛被移植到糖尿病小鼠体内。监测血糖浓度，并进行葡萄糖耐量试验。42天后，胶囊化的胰岛被检索和测定葡萄糖氧化和胰岛素释放rates.Results：有没有差异之间的胶囊含有新鲜或培养的胰岛在其能力，以降低血糖浓度的收件人或在体外功能后胶囊检索。有趣的是，小鼠腹腔内移植700封装胰岛平均血糖水平远低于11毫米的大部分研究，而相同数量的nonencapsulated胰岛有没有对血糖homeostasis.Conclusions有益的影响：封装胰岛移植后，可以扭转高血糖症的腹膜内网站。当移植非囊化胰岛时，未观察到这种效应。由于3天的培养期并不影响移植的结果封装胰岛有证据表明，更适当的血运重建可以解释为什么新鲜分离的胰岛比培养的胰岛更有效。
Background: Although islet transplantation is a promising method to restore normoglycemia in recipients with diabetes, large numbers of pancreatic islets are still needed. It has been suggested that the use of freshly isolated islets could improve transplantation outcome through better vascular engraftment. Using a technique of microencapsulation, a model where revascularization is not possible, we investigated the importance of revascularization for transplantation outcome.Methods: Either 700 or 350 3-day-cultured or noncultured encapsulated islets were transplanted intraperitoneally into syngeneic mice with alloxan-induced diabetes. In addition, 700 nonencapsulated islets were transplanted to mice with diabetes. Blood glucose concentrations were monitored, and glucose tolerance tests were carried out. After 42 days, the encapsulated islets were retrieved and assayed for glucose oxidation and insulin release rates.Results: There were no differences between capsules containing fresh or cultured islets in their capacity to lower the blood glucose concentration of the recipients or in the in vitro function after capsule retrieval. Interestingly, mice that were intraperitoneally transplanted with 700 encapsulated islets had average blood glucose levels well below 11 mM for most of the study, whereas the same number of nonencapsulated islets had no beneficial effects on the blood glucose homeostasis.Conclusions: Encapsulated islets can reverse hyperglycemia after transplantation to the intraperitoneal site. This effect was not seen when nonencapsulated islets were grafted. Since a 3-day culture period did not influence the outcome of transplantation of encapsulated islets there is evidence to suggest that a more appropriate revascularization may explain why freshly isolated islets are more efficient than cultured islets.