Functional correlation between cell adhesive properties and some cell surface proteins.
Functional correlation between cell adhesive properties and some cell surface proteins.
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DOI：
10.1083/jcb.75.2.464
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发表时间：
1977-11
期刊：
影响因子：
--
通讯作者：
Takeichi M
中科院分区：
文献类型：
--
作者：
Takeichi M
通过各种细胞解离处理，分析和表征了中国仓鼠V79细胞的粘附特性。EDTA、胰蛋白酶+Ca ~（2+）和胰蛋白酶+ EDTA解离的细胞之间的聚集性的比较显示，这些细胞具有两种粘附机制，Ca ~（2+）非依赖性和Ca ~（2+）依赖性。前者不依赖于温度，而后者只发生在生理温度。这两种机制都是胰蛋白酶敏感的，但Ca 2+依赖的机制是由Ca 2+保护的。在形态学研究中，Ca 2+非依赖性粘附似乎是一种简单的细胞凝集或絮凝，而Ca 2+依赖性粘附似乎更具有生理性，伴随着细胞变形，导致相邻细胞之间的接触面积增加。乳过氧化物酶催化的碘化的细胞表面蛋白质显示，几种蛋白质更强烈地标记在细胞中的Ca 2 +-非依赖性钙比细胞中没有该属性。还发现，分子量约为150，000的细胞表面蛋白仅存在于具有Ca 2+依赖性钙依赖性的细胞中。该蛋白的碘化和胰蛋白酶化受到Ca ~（2+）的保护，表明其对Ca ~（2+）具有反应性。每个粘附性能的可能机制进行了讨论，考虑到这些蛋白质与细胞粘附的相关性。
The adhesive properties of Chinese hamster V79 cells were analyzed and characterized by various cell dissociation treatments. The comparisons of aggregability among cells dissociated with EDTA, trypsin + Ca2+, and trypsin + EDTA, revealed that these cells have two adhesion mechanisms, a Ca2+-independent and a Ca2+-dependent one. The former did not depend on temperature, whereas the latter occurred only at physiological temperatures. Both mechanisms were trypsin sensitive, but the Ca2+- dependent one was protected by Ca2+ against trypsinization. In morphological studies, the Ca2+-independent adhesion appeared to be a simple agglutination or flocculation of cells, whereas the Ca2+- dependent adhesion seemed to be more physiological, being accompanied by cell deformation resulting in the increase of contact area between adjacent cells. Lactoperoxidase-catalyzed iodination of cell surface proteins revealed that several proteins are more intensely labeled in cells with Ca2+-independent adhesiveness than in cells without that property. It was also found that a cell surface protein with a molecular weight of approximately 150,000 is present only in cells with Ca2+-dependent adhesiveness. The iodination and trypsinization of this protein were protected by Ca2+, suggesting its reactivity to Ca2+. Possible mechanisms for each adhesion property are discussed, taking into account the correlation of these proteins with cell adhesiveness.