Hormonal effects on the sulfation of sulfated glycoprotein in a particulate fraction of the endometrium of rabbit uterus.
Hormonal effects on the sulfation of sulfated glycoprotein in a particulate fraction of the endometrium of rabbit uterus.
复制标题
激素对兔子宫内膜颗粒部分硫酸化糖蛋白硫酸化的影响。
DOI：
10.1093/oxfordjournals.jbchem.a133382
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发表时间：
1981
影响因子：
2.7
通讯作者：
Z. Yosizawa
中科院分区：
文献类型：
--
作者：
M. Isemura;H. Munakata;Z. Yosizawa
从激素或假手术治疗的卵巢切除家兔子宫内膜刮片中分离出颗粒部分。研究了雌激素和孕激素对来自3 '-[35 S]磷酸腺苷5'-磷酸硫酸盐（PAPS）的[35 S]硫酸盐掺入颗粒部分中的内源性受体的影响。雌激素增加[35 S]硫酸盐的掺入，但孕酮抑制这种作用。链霉蛋白酶消化物的DEAE-Sephadex A-25（Cl型）柱层析的结果表明，用0.9 M NaCl（0.9 Fr）洗脱的组分对激素最敏感。0.9 Fr中的主要组分对粗肝素酶具有抗性，而次要组分对该酶敏感。结合前人的研究结果，认为前者为硫酸化糖肽，后者为硫酸乙酰肝素。本研究的结果表明，PAPS可以作为直接的“活化”硫酸盐供体的硫酸化糖蛋白在颗粒级分的酶的硫酸化，硫酸化是大大刺激预处理的兔与雌激素，和雌激素的作用被孕酮抑制。
A particulate fraction was separated from endometrial scrapings of the uterus of hormone- or sham-treated ovariectomized rabbit. The effects of estrogen and progesterone on the incorporation of [35S]sulfate from 3'-[35S]phosphoadenosine 5'-phosphosulfate (PAPS) into endogenous acceptors in the particulate fraction were investigated. Estrogen increased the incorporation of [35S]sulfate, but progesterone suppressed this effect. The results of DEAE-Sephadex A-25 (Cl-form) column chromatography of the pronase digest of the 35S-labeled substances indicated that the fraction eluted with 0.9 M NaCl (0.9 Fr) was most sensitive to the hormones. The major component in 0.9 Fr was resistant to crude heparinase, whereas the minor component was susceptible to this enzyme. The present observation, together with previous findings, suggested that the former was sulfated glycopeptide and the latter heparan sulfate. The results of the present study indicated that PAPS can serve as the direct "activated" sulfate donor in the enzymatic sulfation of sulfated glycoprotein in the particulate fraction, that the sulfation is greatly stimulated by pre-treatment of the rabbit with estrogen, and that the estrogen effect is suppressed by progesterone.