ATP and phosphocreatine changes in single human muscle fibers after intense electrical stimulation.
ATP and phosphocreatine changes in single human muscle fibers after intense electrical stimulation.
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强烈的电刺激后，人体单根肌肉纤维中的 ATP 和磷酸肌酸发生变化。
DOI：
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发表时间：
1991
影响因子：
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通讯作者：
E. Hultman
中科院分区：
文献类型：
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作者：
K. Söderlund;E. Hultman
研究了短暂无氧运动后腺嘌呤核苷酸和糖酵解中间体的恢复模式。在休息时、循环闭塞的间歇性电刺激后立即（总时间 166 秒）以及恢复 20 秒、60 秒、5 分钟和 15 分钟后，从股外侧肌获取活检。运动后，I 型纤维中单纤维中的 ATP 从 23.9 +/- 1.41 降至 14.2 +/- 3.20，II 型纤维中从 25.01 +/- 1.20 降至 13.1 +/- 2.65 mmol/kg 干肌肉。恢复 15 分钟后，I 型和 II 型纤维中的 ATP 分别重新合成至 95% 和 76%。静息状态下，I 型纤维中的磷酸肌酸 (PCr) 含量为 72.3 +/- 4.50，II 型纤维中的磷酸肌酸含量为 83.3 +/- 9.76 mmol/kg 干肌肉。运动后，两种纤维中的 PCr 都被耗尽。恢复 60 秒后，I 型纤维中的 PCr 含量显着高于 II 型纤维（P 小于 0.05），但氧化恢复 5 分钟后，两种纤维类型中的 PCr 水平相等。运动后 15 分钟，II 型纤维的含量（97.8 mmol/kg 干肌肉）显着高于静息值（P 小于 0.05）。
The recovery pattern of adenine nucleotides and glucolytic intermediates after short-lasting anaerobic exercise was studied. Biopsies were obtained from the vastus lateralis muscle at rest, immediately after intermittent electrical stimulation with occluded circulation (total time 166 s), and after 20 s, 60 s, 5 min, and 15 min of recovery. ATP in single fibers decreased from 23.9 +/- 1.41 to 14.2 +/- 3.20 in type I fibers and from 25.01 +/- 1.20 to 13.1 +/- 2.65 mmol/kg dry muscle in type II fibers postexercise. After 15 min of recovery, ATP was resynthesized to 95 and 76% in type I and type II fibers, respectively. The phosphocreatine (PCr) content in type I fibers at rest was 72.3 +/- 4.50 and 83.3 +/- 9.76 mmol/kg dry muscle in type II fibers. After exercise, PCr was depleted in both fiber types. After the 60 s of recovery the PCr content in type I fibers was significantly higher (P less than 0.05) compared with type II fibers, but, after 5 min of oxidative recovery, the PCr levels were equal in the two fiber types. Fifteen minutes postexercise, type II fibers demonstrated a significantly higher content (97.8 mmol/kg dry muscle) compared with its resting value (P less than 0.05).