IgG and complement receptor expression in children treated by peritoneal dialysis
IgG and complement receptor expression in children treated by peritoneal dialysis
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DOI：
10.1007/s00467-005-1850-8
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发表时间：
2005-08-01
影响因子：
3
通讯作者：
Out, TA
中科院分区：
文献类型：
--
作者：
Bouts, AHM;Davin, JC;Out, TA
接受腹膜透析(PD)治疗的儿童感染风险增加。白细胞(WBC)上的免疫球蛋白G受体(Fc、γR)和补体受体(CRS)在吞噬过程中起重要作用。本文对39例腹膜透析患儿血和腹膜透析液中单核细胞、巨噬细胞和中性粒细胞表面Fc-γ-R和CR的表达进行了研究。分别用FITC标记的CD16(Fc Gamma RIII)、CD32(Fc Gamma RII)、CD64(Fc Gamma RI)、CD11b(CR3)和CD35(CR1)单抗标记外周血和PDE中的WBC，并进行流式细胞仪分析。腹膜细胞的Fc、γ、R和CR阳性细胞百分率均低于血液。另一方面，除CD16外，腹膜巨噬细胞和中性粒细胞上的每细胞受体数量[平均荧光强度(MFI)]高于血液。在PD治疗的第一年，血液和透析液中Fc、Gamma、R和CR的表达没有明显变化。在腹膜炎发作期间，血液中所有受体的MFI仅在单核细胞上增加，CD32除外。PDE中FcγR阳性和CR阳性的巨噬细胞和中性粒细胞的百分比增加，而MFI并没有持续增加。帕金森病患儿腹膜细胞Fc-γR阳性和CR阳性的中性粒细胞和巨噬细胞百分比较低，MFI增加，提示处于激活状态。在腹膜炎期间，血液和腹膜细胞能够上调受体的表达，但可能不会达到最大水平。
Children treated by peritoneal dialysis (PD) are at increased risk of infections. IgG receptors (Fc gamma Rs) and complement receptors (CRs) on white blood cells (WBCs) are important for the phagocytic process. We have investigated Fc gamma R and CR expression on monocytes, macrophages and neutrophils in blood and in peritoneal dialysis effluent (PDE) of 39 PD children. WBCs were isolated from blood and PDE, labelled with FITC-conjugated CD16 (Fc gamma RIII), CD32 (Fc gamma RII), CD64 (Fc gamma RI), CD11b (CR3) and CD35 (CR1) monoclonal antibodies, and analysed by flow cytometry. Peritoneal cells had lower percentages of Fc gamma R-positive or CR-positive cells than blood. On the other hand, the receptor number per cell [mean fluorescence intensity (MFI)] was higher on peritoneal macrophages and neutrophils than blood, except for CD16. The Fc gamma R and CR expression in blood and dialysate did not change significantly during the first year of PD treatment. During a peritonitis episode the MFI of all receptors in blood increased only on monocytes, with the exception of CD32. The percentages of Fc gamma R-positive and CR-positive macrophages and neutrophils in the PDE increased, whereas the MFI did not increase consistently. Peritoneal cells of PD children showed a lower percentage of Fc gamma R-positive and CR-positive neutrophils and macrophages, combined with an increased MFI, indicating a state of activation. Blood and peritoneal cells are capable of up-regulating the receptor expression during peritonitis but probably not to a maximum level.