Co-expression of Foxa.a, Foxd and Fgf9/16/20 defines a transient mesendoderm regulatory state in ascidian embryos
Co-expression of Foxa.a, Foxd and Fgf9/16/20 defines a transient mesendoderm regulatory state in ascidian embryos
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DOI：
10.7554/elife.14692
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发表时间：
2016-06-28
期刊：
影响因子：
7.7
通讯作者：
Yasuo, Hitoyoshi
中科院分区：
文献类型：
--
作者：
Hudson, Clare;Sirour, Cathy;Yasuo, Hitoyoshi
在许多双边胚胎中，核β-连环蛋白(n-β-连环蛋白)促进中胚层而不是外胚层谱系。虽然这可能代表了一个进化的古老的发育过程，但在大多数动物中，nβ-连环素诱导的中内胚层的调控结构仍然难以捉摸。在这里，我们证明，在海鞘胚胎中，三个nβ-连环素转录靶点，Foxa.a，FOXD和Fgf9/16/20，都是正确启动中胚层和内胚层基因调控网络所必需的。相反，这三个因素组合在一起就足以产生中胚层基态，这种基态可以进一步编程为中胚层或内胚层谱系。重要的是，我们证明了这三种因子的组合活性足以将发育中的外胚层细胞重新编程为中胚层。我们的结论是，在海鞘胚胎中，Foxa.a、FOXD和Fgf9/16/20的共同表达决定了瞬时中内胚层的调节状态。
In many bilaterian embryos, nuclear beta-catenin (n beta-catenin) promotes mesendoderm over ectoderm lineages. Although this is likely to represent an evolutionary ancient developmental process, the regulatory architecture of n beta-catenin-induced mesendoderm remains elusive in the majority of animals. Here, we show that, in ascidian embryos, three n beta-catenin transcriptional targets, Foxa.a, Foxd and Fgf9/16/20, are each required for the correct initiation of both the mesoderm and endoderm gene regulatory networks. Conversely, these three factors are sufficient, in combination, to produce a mesendoderm ground state that can be further programmed into mesoderm or endoderm lineages. Importantly, we show that the combinatorial activity of these three factors is sufficient to reprogramme developing ectoderm cells to mesendoderm. We conclude that in ascidian embryos, the transient mesendoderm regulatory state is defined by co expression of Foxa.a, Foxd and Fgf9/16/20.