Migration and colonization patterns of HNK-1-immunoreactive neural crest cells in lamprey and swordtail embryos
Migration and colonization patterns of HNK-1-immunoreactive neural crest cells in lamprey and swordtail embryos
复制标题
DOI：
10.2108/zsj.14.305
复制
发表时间：
1997-04-01
影响因子：
0.9
通讯作者：
Tsuneki, K
中科院分区：
文献类型：
--
作者：
Hirata, M;Ito, K;Tsuneki, K
利用HNK-1单克隆抗体，对包括硬骨鱼在内的多种脊椎动物的神经嵴细胞及其衍生物的迁移和定植模式进行了组织化学分析，分析了布鲁克七鳃鳗（Lampetra reissneri）和剑尾鱼（xiphohorus helleri）神经嵴细胞的迁移和定植模式。我们证明了HNK-1在七鳃鳗和剑尾鱼中识别自然嵴细胞和嵴衍生物的亚群。在七鳃鳗和剑尾胚胎的躯干中，hnk -1阳性细胞主要分布在神经嵴细胞的腹侧和背外侧迁移通路上。在剑尾胚胎中，神经嵴细胞在神经管水平沿颈尾轴普遍分布在腹侧通路，但在脊索水平，它们的迁移仅限于体体中部区域。相比之下，在七鳃鳗中，没有hnk -1免疫反应性神经嵴细胞在鳃袋区域后的轴向水平的腹侧途径中向脊索水平以外的腹侧迁移。神经嵴细胞在七鳃鳗躯干的这种迁移模式可能与七鳃鳗身体组织中缺乏解剖学上独特的交感神经链密切相关，这是七鳃鳗身体组织的特征之一。此外，我们在剑尾和七鳃鳗胚胎中发现神经嵴细胞的背向通路延伸到胚胎背鳍。在七鳃鳗中，背侧细胞（Rohon-Beard细胞）和类似染色质细胞的细胞与HNK-1产生免疫反应。
Migration and colonization patterns of neural crest cells were analyzed histochemically in embryos of the brook lamprey, Lampetra reissneri, and the swordtail, Xiphophorus helleri, using HNK-1 monoclonal antibody, which recognizes migratory neural crest cells and crest derivatives in many groups of vertebrates including teleosts. We demonstrated that HNK-1 recognizes a subpopulation of naural crest cells and crest derivatives in the lamprey as well as in the swordtail. In the trunk of lamprey and swordtail embryos, HNK-1-positive cells were observed in the major migratory pathways of neural crest cells, that is ventral and dorso-lateral pathways. In the swordtail embryos, neural crest cells in the ventral pathway were ubiquitously observed along the rostro-caudal axis at the neural tube level, but at the notochord level their migration was restricted to the middle region of the somite. In the lamprey, by contrast, no HNK-1-immunoreactive neural crest cells migrated ventrally beyond the notochord level in the ventral pathway at axial levels behind the gill pouch region. This migration pattern of neural crest cells in the lamprey trunk might be closely related to the absence of anatomically distinct, sympathetic chains in the lamprey, which is one of characteristic features of the lamprey's body organization. Furthermore, we found dorsal pathways, which extended to the embryonic dorsal fin, of neural crest cells in both swordtail and lamprey embryos. In the lamprey, dorsal cells (Rohon-Beard cells) and cells that resemble chromaffin cells immunoreacted with HNK-1.