Delineation of the intronless nature of the genes for the human and hamster beta 2-adrenergic receptor and their putative promoter regions.
Delineation of the intronless nature of the genes for the human and hamster beta 2-adrenergic receptor and their putative promoter regions.
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人类和仓鼠β2-肾上腺素能受体基因的无内含子性质及其推定的启动子区域的描述。
DOI：
10.1016/s0021-9258(18)48239-7
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
R. Lefkowitz
中科院分区：
文献类型：
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作者：
B. Kobilka;T. Frielle;H. Dohlman;M. Bolanowski;R. A. Dixon;P. Keller;M. G. Caron;R. Lefkowitz
β 2-肾上腺素能受体是第一个被克隆的腺苷酸环化酶偶联受体。我们在这里提供了其完整的基因在人类和仓鼠揭示了几个不寻常的和挑衅性的功能的详细表征。这些基因存在于单拷贝中，是无内含子的，并且由同源的18-bp（碱基对）直接重复序列限定。这些发现表明β 2-肾上腺素能受体可能是作为另一个相关基因的加工基因而出现的。在降低的严格性下进行的基因组Southern印迹实际上揭示了额外的弱信号。人类和仓鼠基因序列5 '到转录起始的主要位点是高度同源的，并且共享管家基因的启动子的许多特征。此外，在人类基因组中存在长的（777 bp）开放阅读框，其与β-肾上腺素能受体编码区在框内，并且其仅在受体的起始甲硫氨酸的5 '端234 bp处结束。发现了一种不寻常的cDNA，由一个假定的第二个5 '启动子转录，该启动子含有β-肾上腺素能受体的5'端一半以及受体编码区的5 '端1065-bp，包括整个上游长开放阅读框（足以编码Mr约为28，000的假定蛋白质）。
The beta 2-adrenergic receptor is the first adenylate cyclase-coupled receptor to be cloned. We provide here a detailed characterization of its complete gene in both the human and hamster which reveals several unusual and provocative features. The genes are present in a single copy, are intronless, and are bounded by homologous 18-bp (base pair) direct repeats. These findings suggest that the beta 2-adrenergic receptor may have arisen as a processed gene for another related gene. Genomic Southern blots done at reduced stringency in fact reveal additional weak signals. The human and hamster gene sequences 5' to the principal site of transcription initiation are highly homologous and share many characteristics of promoters for housekeeping genes. Moreover, there is present in the human genome a long (777 bp) open reading frame which is in frame with the beta-adrenergic receptor coding block and which ends only 234 bp 5' to the initiator methionine of the receptor. An unusual cDNA has been found, transcribed from a putative second more 5' promoter which contains the 5' half of the beta-adrenergic receptor as well as 1065-bp 5' to the receptor coding region, including the entire upstream long open reading frame (sufficient to encode a putative protein of Mr approximately 28,000).