Chronic ethanol exposure increases voluntary home cage intake in adult male, but not female, Long-Evans rats.
Chronic ethanol exposure increases voluntary home cage intake in adult male, but not female, Long-Evans rats.
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DOI：
10.1016/j.pbb.2015.10.016
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
McCool BA
中科院分区：
文献类型：
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作者：
Morales M;McGinnis MM;McCool BA
目前的实验研究了10天的慢性间歇性乙醇（CIE）暴露对焦虑样行为和家庭笼乙醇摄入量的影响，使用20%间歇访问（M，W，F）的范例，在男性和女性Long-Evans大鼠。在临床前模型中，从酒精依赖中戒断有助于人类复发，并增加焦虑样行为和自愿酒精消费。我们的实验室已经表明，10天的CIE暴露产生的行为和神经生理学的变化与退出雄性大鼠，但是，我们还没有检查这种暴露制度对女性的乙醇摄入量的影响。在基线期间，女性比男性消耗更多的乙醇，但与男性不同的是，没有显示摄入量的上升。然后将大鼠暴露于CIE，并再次间歇性接触20%乙醇。与基线相比，CIE男性增加了他们的摄入量，而暴露于空气中的男性则没有。雌性动物的乙醇摄入量不受CIE暴露的影响。值得注意的是，两种性别在十字迷宫中表现出显著升高的戒断相关焦虑样行为。最后，大鼠腹腔注射大麻素CB 1受体拮抗剂SR 141716 A（0、1、3、10 mg/kg，i. p.）这减少了男女的酒精摄入量。然而，女性似乎对较低剂量的这种CB 1受体拮抗剂更敏感。我们的研究结果表明，女性消耗更多的乙醇比男性，但是，他们没有升级他们的摄入量使用间歇性访问范例。与男性不同，CIE暴露对女性饮酒没有影响。这是可能的，女性可能比男性更敏感，酒精诱导的饮酒量增加后，短期CIE曝光。最后，我们的研究结果表明，男性和女性可能有不同的药理敏感性CB 1受体阻断乙醇摄入量，至少在目前的条件下。
The current experiment examined the effects of 10 days of chronic intermittent ethanol (CIE) exposure on anxiety-like behavior and home cage ethanol intake using a 20% intermittent access (M, W, F) paradigm in male and female Long-Evans rats. Withdrawal from alcohol dependence contributes to relapse in humans and increases in anxiety-like behavior and voluntary ethanol consumption in preclinical models. Our laboratory has shown that 10 days of CIE exposure produces both behavioral and neurophysiological alterations associated with withdrawal in male rats; however, we have yet to examine the effects of this exposure regime on ethanol intake in females. During baseline, females consumed more ethanol than males but, unlike males, did not show escalations in intake. Rats were then exposed to CIE and were again given intermittent access to 20% ethanol. CIE males increased their intake compared to baseline, whereas air-exposed males did not. Ethanol intake in females was unaffected by CIE exposure. Notably, both sexes expressed significantly elevated withdrawal-associated anxiety-like behavior in the plus maze. Finally, rats were injected with the cannabinoid CB1 receptor antagonist, SR141716A (0, 1, 3, 10 mg/kg, i.p.) which reduced ethanol intake in both sexes. However, females appear to be more sensitive to lower doses of this CB1 receptor antagonist. Our results show that females consume more ethanol than males; however, they did not escalate their intake using the intermittent access paradigm. Unlike males, CIE exposure had no effect on drinking in females. It is possible that females may be less sensitive than males to ethanol-induced increases in drinking after a short CIE exposure. Lastly, our results demonstrate that males and females may have different pharmacological sensitivities to CB1 receptor blockade on ethanol intake, at least under the current conditions.