Distribution, position and genomic characteristics of crossovers in tomato recombinant inbred lines derived from an interspecific cross between Solanum lycopersicum and Solanum pimpinellifolium
Distribution, position and genomic characteristics of crossovers in tomato recombinant inbred lines derived from an interspecific cross between Solanum lycopersicum and Solanum pimpinellifolium
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DOI：
10.1111/tpj.13406
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发表时间：
2017-02-01
期刊：
影响因子：
7.2
通讯作者：
Peters, Sander A.
中科院分区：
文献类型：
--
作者：
Demirci, Sevgin;van Dijk, Aalt D. J.;Peters, Sander A.
我们确定了交叉（CO）的分布，频率和基因组序列参与种间减数分裂重组，通过使用父母指定的变异体的52 F-6重组自交系之间的番茄，番茄，和它的野生亲戚，茄pimpinellifolium。种间CO频率比报道的种内番茄杂交低80%。我们检测到的区域显示出相对较高和较低的CO频率，即所谓的热和冷区域。冷区在很大程度上与异染色质一致，尽管我们在常染色质中发现了有限数量的较小冷区。CO频率在染色体的远端比在着丝粒周围区域和短臂常染色质中更高。常染色质中检测到CO的热点区域，并且CO比预期的偶然更多地位于基因的非翻译区附近的非编码区。除了过度代表CCN重复，我们检测到多聚A/T和AT丰富的图案丰富的1-kb启动子区域侧翼的CO网站。CO位点上最丰富的序列基序与转录因子结合位点（如C2 H2锌指因子类和MADS盒因子）具有较弱的相似性，而InterPro扫描检测到可能参与DNA断裂修复的基因富集。
We determined the crossover (CO) distribution, frequency and genomic sequences involved in interspecies meiotic recombination by using parent-assigned variants of 52 F-6 recombinant inbred lines obtained from a cross between tomato, Solanum lycopersicum, and its wild relative, Solanum pimpinellifolium. The interspecific CO frequency was 80% lower than reported for intraspecific tomato crosses. We detected regions showing a relatively high and low CO frequency, so-called hot and cold regions. Cold regions coincide to a large extent with the heterochromatin, although we found a limited number of smaller cold regions in the euchromatin. The CO frequency was higher at the distal ends of chromosomes than in pericentromeric regions and higher in short arm euchromatin. Hot regions of CO were detected in euchromatin, and COs were more often located in non-coding regions near the 5 untranslated region of genes than expected by chance. Besides overrepresented CCN repeats, we detected poly-A/T and AT-rich motifs enriched in 1-kb promoter regions flanking the CO sites. The most abundant sequence motifs at CO sites share weak similarity to transcription factor-binding sites, such as for the C2H2 zinc finger factors class and MADS box factors, while InterPro scans detected enrichment for genes possibly involved in the repair of DNA breaks.