HIGH AND LOW AFFINITY [H-3]IMIPRAMINE BINDING-SITES IN CONTROL AND PARKINSONIAN BRAINS
HIGH AND LOW AFFINITY [H-3]IMIPRAMINE BINDING-SITES IN CONTROL AND PARKINSONIAN BRAINS
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DOI：
10.1016/0014-2999(85)90473-x
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发表时间：
1985-01-01
影响因子：
5
通讯作者：
AGID, Y
中科院分区：
文献类型：
--
作者：
CASH, R;RAISMAN, R;AGID, Y
[3H]在对照和帕金森病受试者死后大脑的前额皮质和壳核中研究了丙咪嗪的结合。饱和和抑制曲线显示与血清素摄取机制相关的高亲和力[3H]丙咪嗪结合和不依赖于钠且与血清素摄取无关的低亲和力结合。亚细胞分级分离后，高亲和力[3H]丙咪嗪结合位点在突触体组分中富集。在帕金森病中，大脑血清素浓度降低，前额皮质和壳核中高亲和力结合的密度显着降低，而低亲和力结合位点的特征保持不变。对帕金森病患者的壳核进行亚细胞分离后，[3H]丙咪嗪结合的减少主要出现在突触体部分。这些结果与高亲和力[3H]丙咪嗪结合位点和神经元血清素摄取机制之间的关系一致。 [3H]丙咪嗪结合的估计可用作研究人类死后材料中血清素能神经支配的特异性标记。帕金森病患者大脑皮质和皮质下区域中发现的三环类抗抑郁药结合位点密度的降低可能在某种程度上与患者常见的抑郁症有关。
[3H]Imipramine binding was studied in the prefrontal cortex and putamen of post-mortem brains from control and Parkinsonian subjects. Saturation and inhibition curves showed both high affinity [3H]imipramine binding related to the serotonin uptake mechanism and low affinity binding which was sodium-independent and unrelated to serotonergic uptake. After subcellular fractionation, high affinity [3H]imipramine binding sites were enriched in synaptosomal fractions. In Parkinson''s disease, where brain serotonin concentrations are decreased, there was a significant reduction in the density of the high affinity binding in the prefrontal cortex and putamen while the characteristics of the low affinity binding sites remained unchanged. After subcellular fractionation of the putamen of Parkinsonian patients, the decrease in [3H]imipramine binding was found predominantly in the synaptosomal fractions. These results are consistent with a relation between the high affinity [3H]imipramine binding sites and the neuronal serotonin uptake mechanism. Estimation of [3H]imipramine binding could be used as a specific marker for the study of serotonergic innervation in human post-mortem material. The reduction in the density of tricyclic antidepressant binding sites found in cortical and subcortical areas of Parkinsonian brains may be somehow implicated in the depression often seen in patients.