The Combinations Chitosan-Pam3CSK4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc
The Combinations Chitosan-Pam3CSK4 and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for Anticaries Vaccine PAc
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壳聚糖-Pam(3)CSK(4) 和壳聚糖-单磷酰脂质 A 的组合：有望成为防龋疫苗 PAc 的免疫增强佐剂
DOI：
10.1128/iai.00651-19
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发表时间：
2019-12-01
影响因子：
3.1
通讯作者：
Fan, Mingwen
中科院分区：
文献类型：
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作者：
Bi, Yongli;Xu, Qingan;Fan, Mingwen
我们以前证明，重组蛋白PAc可以作为防龋疫苗。然而，PAc相对较弱的免疫原性限制了其应用。在本研究中，我们研究了两种佐剂组合壳聚糖+Pam（3）CSK（4）（chitosan-Pam（3）CSK（4））和壳聚糖+单磷酰脂质A（chitosan-MPL）在体内和体外对PAc蛋白的免疫应答中的作用。PAC-壳聚糖-Pam（3）CSK（4）或PAC-壳聚糖-MPL均能显著提高血清和唾液中PAC特异性抗体滴度，抑制变形链球菌在牙面的定植，具有更好的保护效果，且龋活性显著低于单独使用PAC。壳聚糖-Pam（3）、CSK（4）和壳聚糖-MPL没有显示出统计学显着差异。总之，我们的研究表明，壳聚糖-PAM（3）CSK（4）和壳聚糖-MPL组合物是有希望的防龋疫苗的发展。
We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam(3)CSK(4) (chitosan-Pam(3)CSK(4)) and of chitosan plus monophosphoryl lipid A (chitosan-MPL) in the immune responses to the PAc protein in vivo and in vitro. PAc-chitosan-Pam(3)CSK(4) or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans colonization onto the tooth surfaces, and endowed better protection effect with significantly less caries activities than PAc alone. Chitosan-Pam(3)CSK(4) and chitosan-MPL showed no statistically significant differences. In conclusion, our study demonstrated that the chitosan-Pam(3)CSK(4) and chitosan-MPL combinations are promising for anticaries vaccine development.