THE EFFECTS OF COLONY-STIMULATING FACTORS ON HUMAN MONOCYTE CELL-FUNCTION
THE EFFECTS OF COLONY-STIMULATING FACTORS ON HUMAN MONOCYTE CELL-FUNCTION
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DOI：
10.1016/0192-0561(95)00025-w
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发表时间：
1995-05-01
期刊：
影响因子：
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通讯作者：
NARULA, SK
中科院分区：
文献类型：
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作者：
BOBER, LA;GRACE, MJ;NARULA, SK
我们使用一组功能试验来直接比较GM-CSF和M-CSF对与细胞介导的免疫防御相关的单核细胞活性的模式和效力。GM-CSF和M-CSF在体外刺激人类单核细胞功能的能力和单核细胞激活模式上是相同的。在一组供体中，两种csf在相同浓度下均能有效诱导单核细胞趋化fMLP或LTB(4)。GM-CSF和M-CSF在诱导热杀酵母单核细胞吞噬和调节己糖-单磷酸分流（NET还原）方面表现出相同的效力。两者在防止类固醇（地塞米松）诱导的单核细胞抗细菌（白色念珠菌）和抗真菌（金黄色葡萄球菌）吞噬能力的抑制方面也被发现是相同的。在较低的E:T比下，GM-CSF在刺激白色念珠菌单核细胞杀伤方面比M-CSF更有效。
We used a panel of functional assays to compare directly the pattern and potency of GM-CSF and M-CSF on monocyte activity associated with cell-mediated immune defense. GM-CSF and M-CSF were found to be equivalent both in their capacity to stimulate human monocyte functions in vitro and in their pattern of monocyte activation. The two CSFs were effective in inducing monocyte chemotaxis towards either fMLP or LTB(4) at equivalent concentrations across a panel of donors. GM-CSF and M-CSF demonstrated equipotency in the induction of monocyte phagocytosis of heat-killed baker's yeast and in the regulation of the hexose-monophosphate shunt (NET reduction). Both were also found to be equivalent in preventing steroid (dexamethasone)-induced suppression of monocyte anti-bacterial (Candida albicans) and anti-fungal (Staphylococcus aureus) phagocytic capacities. GM-CSF was somewhat more effective than M-CSF in stimulating monocyte C. albicans killing at a lower E:T ratio.